| 血清和尿液中外泌体miRNAs的表达水平对肾细胞癌的诊断价值 |
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| 引用本文： | 张红森,郭国营,黄秋梅,杨 翠.血清和尿液中外泌体miRNAs的表达水平对肾细胞癌的诊断价值[J].现代肿瘤医学,2022,0(15):2786-2792. |
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| 作者姓名： | 张红森 郭国营 黄秋梅 杨 翠 |
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| 作者单位： | 1.新乡市中心医院/新乡医学院第四临床学院泌尿外科，河南 新乡 453000;
2.新乡医学院第一临床学院血液内科，河南 卫辉 453100 |
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| 基金项目： | 河南省医学科技攻关计划联合共建项目（编号:2018020934） |
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| 摘 要： | 目的:研究血清和尿液中外泌体miRNAs的表达水平对肾细胞癌(RCC)的诊断价值。方法:选择本院2018年11月至2020年08月诊治的68例RCC患者（RCC组）进行前瞻性分析，并将RCC患者根据临床分期进行分组，以本院同期收治的60例肾脏良性病变患者作为对照组。检测患者血清和尿液中外泌体miRNAs相对表达量，分析血清和尿液中外泌体miRNAs在肾细胞癌中的诊断价值。结果:RCC组患者血清、尿液中miR-210、miR-21、miR-153、miR-1233和miR-221表达量均明显高于对照组，miR-34a表达量均明显低于对照组（P＜0.05）；血清中ROC曲线显示AUC最大的为miR-221，其诊断敏感度为79.40%，特异度为95.00%；尿液中最大的为miR-34a，其敏感度为85.30%，特异度为88.30%；不同临床分期RCC患者的血清miR-210、miR-153表达量以及尿液miR-153表达量无显著差异（P＞0.05），但血清和尿液中的miR-21、miR-34a、miR-1233、miR-221表达量以及尿液miR-210表达量在不同分期RCC患者中存在显著差异（P＜0.05）；血清中，miR-210、miR-153与临床分期无相关性（P＞0.05），miR-21、miR-1233、miR-221与临床分期呈正相关，miR-34a与临床分期呈负相关（P＜0.05）；尿液中，miR-210、miR-153与临床分期无相关性（P＞0.05），miR-21、miR-1233、miR-221与临床分期呈正相关，miR-34a与临床分期呈负相关（P＜0.05）。结论:RCC患者血清、尿液外泌体miR-210、miR-21、miR-34a、miR-153、miR-1233和miR-221表达量较良性肾脏病变患者存在显著差异，同时miR-21、miR-34a、miR-1233、miR-221表达量均与RCC患者病理分期存在显著相关性，可为疾病进展评估提供参考。
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| 关 键 词： | 血清 尿液 外泌体miRNAs 肾细胞癌 诊断 |
The diagnostic value of serum and urine exosomal miRNAs expression level in renal cell carcinoma tissues |
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| ZHANG Hongsen,GUO Guoying,HUANG Qiumei,YANG Cui.The diagnostic value of serum and urine exosomal miRNAs expression level in renal cell carcinoma tissues[J].Journal of Modern Oncology,2022,0(15):2786-2792. |
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| Authors: | ZHANG Hongsen GUO Guoying HUANG Qiumei YANG Cui |
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| Affiliation: | 1.Department of Urology,the Xinxiang Central Hospital,the Fourth Affiliated Hospital of Xinxiang Medical University,Henan Xinxiang 453000,China;2.Department of Hematology,the First Affiliated Hospital of Xinxiang Medical University,Henan Weihui 453100,China. |
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| Abstract: | Objective:To study the diagnostic value of serum and urine exosomal miRNAs in renal cell carcinoma(RCC) tissues.Methods:68 patients with RCC(RCC group) diagnosed and treated in the hospital from November 2018 to August 2020 were selected for prospective analysis.They were grouped according to the clinical stage,and 60 patients with benign renal lesions were selected as the control group.The relative expression levels of serum and urine exosomal miRNAs were detected,and their diagnostic value in RCC was analyzed.Results:The expression levels of miR-210,miR-21,miR-153,miR-1233 and miR-221 in serum and urine of RCC group were significantly higher than those in the control group,and the expression level of miR-34a was significantly lower than that in the control group(P＜0.05).The ROC curve showed that among the serum indexes,the AUC of miR-221 was the largest,the diagnostic sensitivity and specificity were 79.40% and 95.00%.Among the urine indexes,the AUC of miR34a was the largest,the sensitivity and specificity were 85.30% and 88.30%.There were no significant differences in expression levels of serum miR-210,miR-153 and urine miR-153 in patients with different clinical stages of RCC(P＞0.05),but the expression levels of serum miR-21,miR-34a,miR-1233 and miR-221,and urine miR-210 showed significant differences in patients with different stages of RCC(P＜0.05).Among serum indexes,there was no correlation between miR-210,miR-153 and clinical stage(P＞0.05),miR-21,miR-1233 and miR-221 were positively correlated with clinical stage,miR-34a was negatively correlated with clinical stage(P＜0.05).Among urine indexes,there was no correlation between miR-210,miR-153 and clinical stage(P＞0.05),miR-21,miR -1233,and miR-221 were positively correlated with clinical stage,miR-34a was negatively correlated with clinical stage(P＜0.05).Conclusion:The expression levels of serum and urine exosomes miR-210,miR-21,miR-34a,miR-153,miR-1233 and miR-221 in patients with RCC are significantly different from those in patients with benign renal lesions.The expression of miR-21,miR-1233,miR-221 and miR-34a is a significantly related to the pathological stage of RCC,which provides reference for the assessment of disease progression. |
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| Keywords: | serum urine exosomal miRNAs renal cell carcinoma diagnosis |
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