| POLD1基因突变及蛋白表达在胃癌组织中的临床意义 |
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| 引用本文： | 阮细玲,吴传智,翁阳,黄幼生,吴琼,徐恒.POLD1基因突变及蛋白表达在胃癌组织中的临床意义[J].现代肿瘤医学,2022,0(5):843-847. |
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| 作者姓名： | 阮细玲 吴传智 翁阳 黄幼生 吴琼 徐恒 |
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| 作者单位： | 1.海南医学院第一附属医院病理科，海南 海口 570102;
2.海口市第四人民医院急诊科，海南 海口 571101;
3.广西医科大学医学科学实验中心，广西 南宁 530021 |
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| 基金项目： | 国家自然科学基金(编号:82060437)。 |
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| 摘 要： | 目的:观察DNA聚合酶δ(polymerase data,Polδ)的催化亚基p125与胃癌细胞恶性增殖的关系,并探讨p125的编码基因POLD1与胃癌发生、发展的关系.方法:采用免疫组化法检测胃癌组织及癌旁组织中p125的表达;沉默胃癌细胞中POLD1基因,用MTT实验检测POLD1基因沉默前后胃癌细胞的增殖速率.采...
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| 关 键 词： | 胃癌 p125 POLD1 细胞增殖 测序 |
Clinical significance of POLD1 gene mutation and protein expression in gastric carcinoma |
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| RUAN Xiling,WU Chuanzhi,WENG Yang,HUANG Yousheng,WU Qiong,XU Heng.Clinical significance of POLD1 gene mutation and protein expression in gastric carcinoma[J].Journal of Modern Oncology,2022,0(5):843-847. |
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| Authors: | RUAN Xiling WU Chuanzhi WENG Yang HUANG Yousheng WU Qiong XU Heng |
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| Affiliation: | 1.Department of Pathology,the First Affiliated Hospital of Hainan Medical College,Hainan Haikou 570102,China;2.Department of Emergency,the Fourth People's Hospital,Hainan Haikou 571101,China;3.Department of Medical Scientific Experimental Research Center |
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| Abstract: | Objective:To investigate the relationship between the catalytic subunit p125 of DNA polymerase data(Pol δ) and the malignant proliferation of gastric cancer cells, and to explore the relationship between POLD1 gene and the occurrence and development of gastric carcinoma.Methods:The expression of p125 in gastric carcinoma and its peritumoral tissues was detected by immunohistochemical method.POLD1 gene was silenced in gastric carcinoma cells, and the proliferation rate of gastric cancinoma cells before and after POLD1 gene silencing was measured by MTT Assay.The cDNA of POLD1 gene in gastric cancinoma cells was sequenced by sanger method.Results:Immunohistochemical staining results showed that the positive rate of p125 expression in gastric carcinoma was significantly higher than that in peritumoral tissue.The results of MTT assay showed that the proliferation rate of POLD1 gene silenced gastric carcinoma cells was significantly lower than that of control cells.Sanger’s sequence analysis showed that there were 4 point mutations, 1 base insertion and 1 base deletion in the cDNA of POLD1 gene in gastric carcinoma cells.Conclusion:Over-expression of p125 is associated with malignant proliferation of gastric carcinoma.The point mutation and base insertion of POLD1 gene in gastric carcinoma cells result in the alteration of its amino acid sequence, which may be one of the molecular mechanisms leading to the overexpression of p125 and the malignant proliferation of gastric carcinoma cells. |
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| Keywords: | gastric carcinoma p125 POLD1 cell proliferation sequencing |
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