| Her2/ECD-sf162/TM融合杆状病毒表达及鉴定 |
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| 引用本文： | 李谨革,龙 敏,王 希,董 柯,林 芳,刘 冲,张惠中.Her2/ECD-sf162/TM融合杆状病毒表达及鉴定[J].现代肿瘤医学,2015,0(13):1798-1802. |
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| 作者姓名： | 李谨革 龙 敏 王 希 董 柯 林 芳 刘 冲 张惠中 |
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| 作者单位： | 第四军医大学唐都医院临床实验与检验科，陕西 西安 710038 |
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| 基金项目： | 国家自然科学基金资助项目（编号:81172148） |
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| 摘 要： | 目的:获得融合表达的Her2/ECD-sf162/TM杆状病毒，为后续基于SIV Gag蛋白的Her2病毒样颗粒疫苗的制备奠定实验基础。方法:将Her2胞外段基因与猴免疫缺陷病毒（simian immunodeficiency virus，SIV）包膜蛋白sf162跨膜区基因融合的Her2/ECD-sf162/TM pFastBac重组表达载体，转座大肠杆菌E.coli DH10Bac菌株，获得重组杆粒，在昆虫细胞中表达获得重组杆状病毒，Western blot方法对该病毒进行鉴定。结果:构建的Her2/ECD-sf162/TM杆状病毒表达载体经PCR鉴定正确，转染昆虫细胞后获得了重组杆状病毒，该融合杆状病毒蛋白可与抗Her2抗体发生免疫反应。结论:成功构建的Her2/ECD-sf162/TM杆状病毒可用于Her2病毒样颗粒疫苗的制备。
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| 关 键 词： | Her2/neu 胞外区 SIV 杆状病毒 |
Expression and detection of Her2/ECD -sf162/TM fusion peptide with baculovirus ex-pression system |
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| Li Jin' ge,Long Min,Wang Xi,Dong Ke,Lin Fang,Liu Chong,Zhang Huizhong.Expression and detection of Her2/ECD -sf162/TM fusion peptide with baculovirus ex-pression system[J].Journal of Modern Oncology,2015,0(13):1798-1802. |
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| Authors: | Li Jin' ge Long Min Wang Xi Dong Ke Lin Fang Liu Chong Zhang Huizhong |
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| Affiliation: | Department of Clinical Research and Diagnosis,Tangdu Hospital,Fourth Military Medical University,Shaanxi Xi'an 710038，China. |
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| Abstract: | Objective:To gain Her2/ECD-sf162/TM recombinant baculovirus，laiding a foundation for further study of Her2 virus like particles vaccine that based on SIV Gag protein.Methods:Her2/ECD-sf162/TM fusion gene recombinant pFastBac-1 expression vector of Her2 extracellular domain and SIV envelope protein sf162 transmembrane domain was constructed，and then transformed into E.coli strain DH10Bac to gain recombinant bacmids，which then were transfected into insect cells to express recombinant baculovirus with bac-to-bac systerm.In turn，Western blot were performed identificated the recombination.Results:Recombinant pFastBac-1 expression vector was successfully constructed and high titer recombinant baculovirus which could imunoreacted with Her2 antibody were gained.Conclusion:Her2/ECD-sf162/TM recombinant baculovirus can be used to develop Her2 virus like particles vaccine. |
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| Keywords: | Her2/neu extracellular domain SIV baculovirus |
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