| 肺癌A549细胞诱导RAW264.7破骨细胞分化中AMPK信号通路的作用 |
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| 引用本文： | 赵雪强,蒋碧佳,银建华,卢春兰,林 云.肺癌A549细胞诱导RAW264.7破骨细胞分化中AMPK信号通路的作用[J].现代肿瘤医学,2021,0(17):2952-2955. |
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| 作者姓名： | 赵雪强 蒋碧佳 银建华 卢春兰 林 云 |
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| 作者单位： | 桂林医学院第二附属医院呼吸与危重症医学科,广西 桂林 541199 |
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| 基金项目： | 广西教育厅高校中青年教师基础能力提升项目(编号:2017KY0499)；广西卫计委适宜技术开发与推广应用项目(编号:S2017107)；桂林医学院中青年教师课题（编号:2018glmcy101） |
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| 摘 要： | 目的:探讨AMPK信号通路在体外肺癌细胞诱导破骨细胞分化中的作用。方法:将肺癌A549细胞与RAW264.7细胞共培养后随机分为阴性对照组、阳性对照组、AICAR组，药物干预后行TRAP染色观察破骨细胞的分化，流式细胞仪观察破骨细胞细胞凋亡情况，qPCR检测破骨细胞AMPK、mTOR和CTSK mRNA的表达，Western blot检测破骨细胞AMPK、p-AMPK、mTOR和p-mTOR蛋白的表达。结果:与阴性对照组比较，AICAR组破骨细胞数明显减少，差异具有统计学意义（P＜0.05）；对诱导破骨细胞凋亡的作用无差异；上调AMPK的mRNA和蛋白表达，下调mTOR的mRNA和蛋白表达，差异具有统计学意义（P＜0.05）。结论:AICAR可以抑制肺癌细胞诱导破骨细胞分化，而AMPK/mTOR信号通路可能参与了肺癌细胞诱导破骨细胞分化过程。
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| 关 键 词： | 肺癌 破骨细胞分化 AMPK 骨转移 |
Study of the effect of AMPK signaling pathway on RAW264.7 cells osteoclast differentiation induced by lung cancer A549 cells |
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| ZHAO Xueqiang,JIANG Bijia,YIN Jianhua,LU Chunlan,LIN Yun.Study of the effect of AMPK signaling pathway on RAW264.7 cells osteoclast differentiation induced by lung cancer A549 cells[J].Journal of Modern Oncology,2021,0(17):2952-2955. |
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| Authors: | ZHAO Xueqiang JIANG Bijia YIN Jianhua LU Chunlan LIN Yun |
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| Affiliation: | Department of Pulmonary and Critical Care Medicine，the Second Affiliated Hospital of Guilin Medical College，Guangxi Guilin 541199，China. |
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| Abstract: | Objective:To study the role of AMPK signaling pathway in osteoclast differentiation induced by lung cancer cells in vitro.Methods:The lung cancer A549 cells were co-cultured with RAW264.7 and randomly divided into a negative control group,a positive control group,and an AICAR group.After drug intervention,TRAP staining was performed to observe osteoclasts apoptosis,qPCR was performed to detect the expression of AMPK,mTOR and CTSK mRNA in osteoclasts,and Western blot analysis was used to detect the expression of AMPK,p-AMPK,mTOR and p-mTOR protein in osteoclasts.Results:Compared with the control group,the number of osteoclasts in the AICAR group was significantly reduced,and the expression of AMPK was up-regulated and mTOR was down-regulated.They had a synergistic effect（P＜0.05）.Conclusion:AICAR can inhibit lung cancer cells to induce osteoclast differentiation.AMPK/mTOR signal may be involved in lung cancer cells to induce osteoclast differentiation. |
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| Keywords: | lung cancer osteoclast differentiation AMP-activated protein kinase bone metastasis |
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