| 小白菊内酯对甲状腺癌TPC-1细胞增殖、迁移和侵袭的影响及机制 |
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| 引用本文： | 张文婧,王佳贺.小白菊内酯对甲状腺癌TPC-1细胞增殖、迁移和侵袭的影响及机制[J].现代肿瘤医学,2021,0(2):185-189. |
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| 作者姓名： | 张文婧 王佳贺 |
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| 作者单位： | 中国医科大学附属盛京医院老年病科，辽宁 沈阳 110004 |
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| 基金项目： | 辽宁省自然科学基金指导计划（编号:201602858） |
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| 摘 要： | 目的:研究小白菊内酯（parthenolide,PTL）对甲状腺癌TPC-1细胞增殖、迁移和侵袭的影响及其可能的机制。方法:应用0、2.5、5、10、20 μmol/L的PTL处理甲状腺癌TPC-1细胞24 h，CCK-8法检测不同浓度的PTL对TPC-1细胞增殖的影响；划痕实验和Transwell实验观察PTL对TPC-1细胞迁移和侵袭能力的影响；Western blotting检测E-cadherin、N-cadherin、Vimentin、MMP-9、TGF-β、Smad3、p-Smad3蛋白表达水平的变化。结果:CCK-8实验结果表明，PTL可抑制TPC-1细胞的增殖，并呈一定的浓度依赖性；划痕实验和Transwell实验表明，PTL可减弱TPC-1细胞的迁移和侵袭能力；Western blotting结果显示，PTL可上调E-cadherin的表达量，下调N-cadherin、Vimentin、MMP-9、TGF-β、p-Smad3的表达水平。结论:PTL以浓度依赖的方式抑制甲状腺癌TPC-1细胞增殖；PTL可能通过下调TGF-β的表达和抑制Smad3的磷酸化，逆转上皮间质转化（epithelial-mesenchymal transition,EMT）的发生，从而降低甲状腺癌TPC-1细胞的侵袭、迁移能力。
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| 关 键 词： | 甲状腺癌 小白菊内酯 增殖 侵袭 迁移 EMT |
Effect and mechanism of parthenolide on proliferation,migration and invasion of thyroid cancer TPC-1 cells |
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| ZHANG Wenjing,WANG Jiahe.Effect and mechanism of parthenolide on proliferation,migration and invasion of thyroid cancer TPC-1 cells[J].Journal of Modern Oncology,2021,0(2):185-189. |
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| Authors: | ZHANG Wenjing WANG Jiahe |
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| Affiliation: | Department of Geriatrics,Shengjing Hospital of China Medical University,Liaoning Shenyang 110004,China. |
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| Abstract: | Objective:To study the effect of parthenolide (PTL) on the proliferation,migration and invasion of TPC-1 cells in thyroid cancer and its possible mechanism.Methods:TPC-1 cells were treated with different concentrations of PTL (0,2.5,5,10,and 20 μmol/L) for 24 h,and the effect of on the proliferation was detected by the CCK-8 assay.We then examined the effect of PTL on cell migration and invasion via scratch and Transwell assays,respectively.The protein expression of E-cadherin,N-cadherin,Vimentin,MMP-9,TGF-β,Smad3 and p-Smad3 were detected by Western blotting.Results:CCK-8 assay results showed that the cell proliferation was suppressed in a concentration-dependent manner of PTL.Scratch and Transwell assays demonstrated that PTL significantly suppressed the migration and invasion ability of TPC-1 cells.Western blotting showed the E-cadherin expression was up-regulated,whereas N-cadherin,Vimentin,MMP-9,TGF-β,p-Smad3 expression were down-regulated following treatment with PTL.Conclusion:PTL inhibited cell proliferation of thyroid carcinoma TPC-1 cells in a concentration-dependent manner.PTL may reverse the occurrence of epithelial-mesenchymal transition by down-regulating the expression of TGF-β and inhibiting the phosphorylation of Smad3,reducing the invasion and migration ability of thyroid cancer TPC-1 cells. |
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| Keywords: | thyroid cancer parthenolide proliferation invasion migration EMT |
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