| 顺铂联合1，25(OH)2D3对肺癌细胞凋亡及VEGF、IL-6表达水平的影响 |
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| 引用本文： | 刘洪梅,李安平,刘 静,邱 斌,王希柱,韩素桂.顺铂联合1，25(OH)2D3对肺癌细胞凋亡及VEGF、IL-6表达水平的影响[J].现代肿瘤医学,2018,0(23):3722-3725. |
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| 作者姓名： | 刘洪梅 李安平 刘 静 邱 斌 王希柱 韩素桂 |
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| 作者单位： | 1.唐山市人民医院核医学检验科；2.体检中心；3.胸外科；4.心内科，河北 唐山 063000 |
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| 基金项目： | 河北省医学科学研究重点课题计划（编号:20171287） |
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| 摘 要： | 目的:探讨顺铂联合1，25(OH)2D3对肺癌细胞凋亡及VEGF、IL-6表达水平的影响。方法:体外培养恶性胸水中肺癌细胞，经药物作用后CCK-8法测定细胞抑制率，流式细胞仪检测细胞周期和凋亡，应用化学发光仪检测细胞培养液中VEGF、IL-6表达水平。结果:不同浓度的1，25(OH)2D3、顺铂单独作用于肺癌细胞均有明显的抑制作用，两药联合表现为协同作用，与单药相比具有显著性差异(P<0.05)。流式细胞分析显示，经药物联合处理后的肺癌细胞的G0/G1期细胞数增多，诱导细胞凋亡(P<0.05)。顺铂、1，25(OH)2D3对VEGF的表达均有抑制作用，顺铂能够上调IL-6水平，1，25(OH)2D3对IL-6表达无显著作用。两药联合作用时能抑制VEGF、IL-6的表达，且较单药显著增强(P<0.05)。结论:顺铂联合1，25(OH)2D3能够抑制肺癌细胞生长，诱导细胞凋亡，降低了VEGF、IL-6的表达水平，可为肺癌提供新的治疗策略。
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| 关 键 词： | 1 25(OH)2D3 顺铂 肺癌细胞 凋亡 VEGF IL-6 |
The effect of cisplatin combined with 1，25(OH)2D3 on the apoptosis and the expression of VEGF and IL-6 in lung cancer cells |
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| Liu Hongmei,Li Anping,Liu Jing,Qiu Bin,Wang Xizhu,Han Sugui.The effect of cisplatin combined with 1，25(OH)2D3 on the apoptosis and the expression of VEGF and IL-6 in lung cancer cells[J].Journal of Modern Oncology,2018,0(23):3722-3725. |
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| Authors: | Liu Hongmei Li Anping Liu Jing Qiu Bin Wang Xizhu Han Sugui |
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| Affiliation: | 1.Nuclear Medicine Laboratory;2.Medical Examination Center；3.Department of Thoracic Surgery；4.Department of Cardiology，Tangshan People's Hospital，Hebei Tangshan 063000，China. |
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| Abstract: | Objective:To investigate the effect of cisplatin combined with 1，25(OH)2D3 on the apoptosis and the expression of VEGF and IL-6 in lung cancer cells.Methods:Lung cancer cells from malignant pleural effusion were cultured in vitro.The inhibition rate of tumor cells was measured by CCK-8 assay.The cell cycle and apoptosis were detected by flow cytometry.The expression level of VEGF and IL-6 in cell culture medium was detected by chemiluminescence.Results:Different concentrations of 1，25(OH)2D3 and cisplatin alone had obvious inhibitory effects on lung cancer cells，which increased with time and concentration increased (P<0.05).The combination of the two drugs showed synergistic effect，which was significantly different from that of single drug (P<0.05).Cell cycle analysis showed that the number of G0/G1 cells increased in the lung cancer cells treated by drug treatment and decreased in S and G2/M stages.DDP，1，25(OH)2D3 inhibited the expression of VEGF，DDP could decrease the level of IL-6，and 1，25(OH)2D3 had no significant effect on IL-6 expression.The combination of DDP and 1，25(OH)2D3 significantly induced the expression of VEGF and IL-6，and significantly enhanced (P<0.05) compared with the single drug.Conclusion:DDP combined with 1，25(OH)2D3 can inhibit the growth of lung cancer cells，induce apoptosis and reduce the expression level of VEGF and IL-6，can provide a new therapeutic strategy for lung cancer. |
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| Keywords: | 1 25(OH)2D3 cisplatin lung cancer cell apoptosis vascular endothelial growth factor(VEGF) interleukin-6 (IL-6) |
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