| miR-21沉默下调β-catenin表达对食管癌细胞增殖和凋亡的影响 |
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| 引用本文： | 王荦楠,姬颖华,靳彩玲,寇小格,曲智锋.miR-21沉默下调β-catenin表达对食管癌细胞增殖和凋亡的影响[J].现代肿瘤医学,2018,0(24):3924-3927. |
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| 作者姓名： | 王荦楠 姬颖华 靳彩玲 寇小格 曲智锋 |
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| 作者单位： | 1.新乡医学院第一附属医院肿瘤科,河南 新乡 453100；
2.河南科技大学第一附属医院肿瘤科,河南 洛阳 471000 |
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| 基金项目： | 河南省科技攻关课题(编号:072102310086) |
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| 摘 要： | 目的:探讨miR-21沉默对食管癌细胞增殖和凋亡的影响及其与β-catenin表达的关系。方法:通过脂质体转染法沉默食管癌Eca9706细胞株中的miR-21，采用荧光定量（RT-PCR）检测miR-21、β-catenin mRNA的相对表达水平；蛋白质印迹法（Western blot）检测β-catenin蛋白的相对表达水平。MTT法检测细胞增殖能力，流式细胞仪检测细胞凋亡和细胞周期。结果:转染48 h后，与对照组和NC组相比，miR-21组Eca9706细胞中miR-21、β-catenin mRNA和β-catenin蛋白的相对表达水平均明显降低。MTT检测miR-21组细胞的光密度值（OD值）较对照组和NC组明显下降。与NC组和对照组相比，流式细胞仪检测miR-21组中细胞的凋亡率明显上升，G0/G1期细胞所占比例明显升高，S期细胞所占比例明显下降。结论:沉默miR-21能够通过下调β-catenin的表达来抑制食管癌Eca9706细胞增殖及诱导其凋亡。
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| 关 键 词： | miR-21 食管癌 细胞增殖 细胞凋亡 β-catenin |
Effect of silencing miR-21 downregulation expressions of β-catenin on proliferation and apoptosis of esophageal cancer cells |
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| Wang Luonan,Ji Yinghua,Jin Cailing,Kou Xiaoge,Qu Zhifeng.Effect of silencing miR-21 downregulation expressions of β-catenin on proliferation and apoptosis of esophageal cancer cells[J].Journal of Modern Oncology,2018,0(24):3924-3927. |
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| Authors: | Wang Luonan Ji Yinghua Jin Cailing Kou Xiaoge Qu Zhifeng |
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| Affiliation: | 1.Department of Oncology,First Affiliated Hospital of Xinxiang Medical College,Henan Xinxiang 453100,China;2.Department of Oncology,First Affiliated Hospital of Henan University of Science and Technology,Henan Luoyang 471000,China. |
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| Abstract: | Objective:To explore the influence of miR-21 silence on the proliferation and apoptosis of esophageal cancer cells and the relationship with β-catenin expression.Methods:miR-21 in Eca9706 esophagus carcinoma cell lines was silent by liposome transfection method,and use fluorescence quantitative（RT-PCR) and Western blot methods（Western blot) to detect the relative expression level of miR-21,β-catenin mRNA and β-catenin protein.The proliferation of esophageal cancer cells was detected by the tetramethazole blue method（MTT),and the apoptosis and cell cycle were detected by the flow cytometer.Results:The relative expression levels of miR-21,β-catenin mRNA and β-catenin protein in the miR-21 group of the Eca9706 cells were significantly lower than those of the control group and the NC group after 48 h transfecting.There was a significant decrease in the optical density（OD) of the miR-21 cells group compared to the control group and the NC group which detected by MTT.Flow cytometry test results showed that cell apoptosis rate in miR-21 group increased and the cell proportion of G0/G1 phase increased significantly,and the cell proportion of S stage decreased obviously compared with NC group and the control group.Conclusion:The silent miR-21 was able to inhibit the proliferation of Eca9706 cells and induce the apoptosis by reducing the expression of the β-catenin. |
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| Keywords: | miR-21 esophageal cancer cell proliferation cell apoptosis β-catenin |
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