| 人脐血清源性外泌体对肝癌Hep3B细胞活力的影响 |
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| 引用本文： | 汪庆强,李雯慧,方 诚,范 明,张小晶,唐明尧,付 航,陈 勇.人脐血清源性外泌体对肝癌Hep3B细胞活力的影响[J].现代肿瘤医学,2018,0(20):3173-3176. |
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| 作者姓名： | 汪庆强 李雯慧 方 诚 范 明 张小晶 唐明尧 付 航 陈 勇 |
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| 作者单位： | 1.空军军医大学第一附属医院肝胆外科；2.妇产科，陕西 西安 710032 |
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| 基金项目： | National Natural Science Foundation of China(No.81670563）；国家自然科学基金资助项目(编号:81670563) |
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| 摘 要： | 目的:探究脐血清源性外泌体对肝癌Hep3B细胞活力的影响。方法:提取健康足月胎儿脐血清源性外泌体，加入细胞培养基孵育24小时，MTT检测细胞活力变化，倒置显微镜观察细胞形态变化，流式细胞术检测细胞凋亡，Western blot检测Cleaved-Caspase3表达变化。结果:脐血清源性外泌体能显著抑制肝癌Hep3B细胞活力，导致细胞发生形态学改变、凋亡细胞比例增加及活化的Caspase3水平上升。结论:脐血清源性外泌体能诱导肝癌细胞发生凋亡，具有潜在的抑制肿瘤的生物学活性。
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| 关 键 词： | 人脐血清 外泌体 肝癌 细胞活力 |
The effect of exosomes derived from human umbilical serum on Hep3B cell viability |
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| Wang Qingqiang,Li Wenhui,Fang Cheng,Fan Ming,Zhang Xiaojing,Tang Mingyao,Fu Hang,Chen Yong.The effect of exosomes derived from human umbilical serum on Hep3B cell viability[J].Journal of Modern Oncology,2018,0(20):3173-3176. |
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| Authors: | Wang Qingqiang Li Wenhui Fang Cheng Fan Ming Zhang Xiaojing Tang Mingyao Fu Hang Chen Yong |
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| Affiliation: | 1.Department of Hepatobiliary Surgery;2.Department of Obstetrics and Gynecology，The First Affiliated Hospital of the Air Force Military Medical University,Shaanxi Xi'an 710032,China. |
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| Abstract: | Objective:To investigate the effects of exosomes derived from human umbilical serum on Hep3B cell viability.Methods:Exosomes were isolated from umbilical serum of healthy full-term fetus and were added into cell medium for 24 h.Cell viability was evaluated using MTT assay,cell morphology was observed with invert microscope,cell apoptosis was analyzed by flow cytometry and the level of Cleaved-Caspase3 was detected by Western blot.Results:Exosomes derived from human umbilical serum can suppress Hep3B cell viability significantly,induce cell morphological changes,increase the proportion of apoptotic cells,accompanied with increased level of cleaved-Caspase3.Conclusion:Exosomes isolated from umbilical serum may induce apoptosis of hepatic cancer,acting with the bioactivity of tumor suppressor. |
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| Keywords: | human umbilical serum exosome hepatic cancer cell viability |
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