| 非小细胞肺癌患者Th17细胞相关因子的表达及其临床意义 |
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| 引用本文： | 朱勇,施舜缤,张林,钱涛涛,王海龙.非小细胞肺癌患者Th17细胞相关因子的表达及其临床意义[J].现代肿瘤医学,2021,0(3):413-417. |
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| 作者姓名： | 朱勇 施舜缤 张林 钱涛涛 王海龙 |
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| 作者单位： | 苏州市第九人民医院胸外科，江苏 苏州 215200 |
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| 基金项目： | 苏州市科学技术项目(编号:WS201313)。 |
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| 摘 要： | 目的:通过检测Th17细胞及其相关因子在非小细胞肺癌（non-small cell lung cancer,NSCLC）中的表达，探讨其在NSCLC中的临床意义。方法:选择我院2016年2月至2019年2月收治的41例NSCLC患者纳为NSCLC组，选择同期在我院接受体检的健康人员19例为正常对照组（NC组）。收集NC组受检者外周血及NSCLC患者外周血、癌病灶组织和癌旁组织。采用流式细胞术检测外周血Th17细胞水平；酶联免疫吸附法检测外周血及肺组织匀浆上清液中IL-17水平；免疫组化染色法检测组织中IL-17（IL-17+细胞）水平。对比不同病理类型、病理分期NSCLC患者外周血、癌病灶组织及癌旁组织Th17、IL-17水平。结果:NSCLC组患者外周血Th17细胞及IL-17水平均高于NC组，差异有统计学意义（P<0.05）。免疫组化检测显示IL-17+细胞以细胞质呈棕黄色和（或）棕褐色为阳性表达，癌病灶组织中见典型IL-17+细胞明显散落，成团染为棕褐色。NSCLC患者癌病灶组织Th17、IL-17水平均高于癌旁组织，差异有统计学意义（P<0.05）。不同性别、年龄患者外周血Th17细胞水平差异均无统计学意义（P>0.05）。肺鳞癌和肺腺癌患者外周血Th17及癌病灶组织和癌旁组织中Th17、IL-17水平差异无统计学意义（P>0.05）。Ⅲ-Ⅳ期NSCLC患者外周血、癌病灶组织、癌旁组织Th17、IL-17水平均高于Ⅰ-Ⅱ期，差异有统计学意义（P<0.05）。结论:Th17细胞及其相关因子在NSCLC患者外周血及癌病灶组织中表达明显上调，且随着肿瘤病理分期增加显著升高，可能与NSCLC的发生发展有关。
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| 关 键 词： | NSCLC Th17细胞 相关因子 临床意义 |
The expression and clinical significance of Th17 cell correlation factor in patients with non-small cell lung cancer |
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| ZHU Yong,SHI Shunbin,ZHANG Lin,QIAN Taotao,WANG Hailong.The expression and clinical significance of Th17 cell correlation factor in patients with non-small cell lung cancer[J].Journal of Modern Oncology,2021,0(3):413-417. |
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| Authors: | ZHU Yong SHI Shunbin ZHANG Lin QIAN Taotao WANG Hailong |
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| Affiliation: | Department of Thoracic Surgery，the 9th People's Hospital of Suzhou，Jiangsu Suzhou 215200，China. |
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| Abstract: | Objective:By detecting the expression of Th17 cells and related factors in NSCLC,the clinical significance of Th17 cells in NSCLC was discussed.Methods:41 NSCLC patients admitted to our hospital from February 2016 to February 2019 were selected as the NSCLC group,and 19 healthy people who underwent physical examination in our hospital during the same period were selected as the normal control group(NC group).Peripheral blood,cancerous tissues and adjacent tissues of patients with NSCLC and peripheral blood of peoples in NC group were collected.The level of Th17 cells in peripheral blood was detected by flow cytometry.The level of IL-17 in the homogenate supernatant of peripheral blood and lung tissues was detected by enzyme-linked immunosorbent assay.IL-17(IL-17+cells)in tissues was detected by immunohistochemical staining.The levels of Th17 and IL-17 in peripheral blood,cancerous tissues and para-cancerous tissues of NSCLC patients with different pathological types and pathological stages were compared.Results:The levels of Th17 cells and IL-17 in peripheral blood of patients in NSCLC group were higher than those in NC group,and the difference was statistically significant(P<0.05).Immunohistochemical tests showed that IL-17+cells were positively expressed in cytoplasm with brown-brown color and tan color,and typical IL-17+cells were significantly scattered in the tumor tissues and stained with brown color.The levels of Th17 and IL-17 in cancer tissues of NSCLC patients were higher than those in para-cancerous tissues,and the difference was statistically significant(P<0.05).Th17 and IL-17 levels in peripheral blood of patients with different pathological types of lung squamous cell carcinoma and lung adenocarcinoma,as well as in cancerous tissues and para-cancerous tissues,were not statistically significant(P>0.05).Ⅲ-ⅣNSCLC,focal cancer tissue,tissue adjacent to carcinoma patients peripheral blood Th17,IL-17 levels were higher thanⅠ-Ⅱperiod,and the difference was statistically significant(P<0.05).Conclusion:The expression of Th17 cells and its related factors in peripheral blood and cancer tissues of NSCLC patients was significantly upregaged and increased with the increase of tumor pathological stages,which may be related to the occurrence and development of NSCLC. |
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| Keywords: | NSCLC Th17 cell correlation factor clinical significance |
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