| IFN-γ通过诱导上皮间质转化增强人乳头状甲状腺癌细胞株的侵袭转移能力 |
| |
| 引用本文： | 吕男男,' target='_blank'>,高 芸,单忠艳.IFN-γ通过诱导上皮间质转化增强人乳头状甲状腺癌细胞株的侵袭转移能力[J].现代肿瘤医学,2019,0(6):947-951. |
| |
| 作者姓名： | 吕男男 ' target='_blank'> 高 芸 单忠艳 |
| |
| 作者单位： | 1.沈阳市第四人民医院内分泌科，辽宁 沈阳 110034;2.中国医科大学附属第一医院内分泌科、内分泌研究所，辽宁 沈阳 110001 |
| |
| 基金项目： | 辽宁省自然科学基金指导计划（编号:20180550452） |
| |
| 摘 要： | 目的:观察IFN-γ对人乳头状甲状腺癌细胞株TPC-1侵袭和转移能力的影响，并探讨上皮间质转化过程（EMT）在其中的作用。方法:采用划痕愈合实验和Transwell侵袭实验检测IFN-γ对TPC-1侵袭转移能力的影响；实时定量PCR和Western检测EMT标志物E-cadherin，N-cadherin，Vimentin的mRNA水平及蛋白水平变化；免疫荧光检测上述标志物的定位表达。结果:划痕愈合实验和Transwell侵袭实验结果显示:IFN-γ增强了TPC-1的侵袭转移能力（P＜0.05）,实时定量PCR结果显示EMT的上皮标志物E-cadherin的mRNA表达水平明显下降，间质标志物Vimentin的mRNA表达上调，而N-cadherin的mRNA未见明显变化。Western结果显示E-cadherin在IFN-γ作用下表达下调；N-cadherin与Vimenin表达上升；免疫荧光结果与Western结果一致。结论:INF-γ可以通过诱导EMT，促进TPC-1侵袭转移，提示炎性介质IFN-γ可能参与人乳头状甲状腺癌的侵袭转移。
|
| 关 键 词： | IFN-γ 侵袭转移 乳头状甲状腺癌细胞 EMT |
IFN-γ facilitates malignant progression in PTC cell line by inducing EMT |
| |
| Lv Nannan,' target='_blank'>,Gao Yun,Shan Zhongyan.IFN-γ facilitates malignant progression in PTC cell line by inducing EMT[J].Journal of Modern Oncology,2019,0(6):947-951. |
| |
| Authors: | Lv Nannan ' target='_blank'> Gao Yun Shan Zhongyan |
| |
| Affiliation: | 1.Department of Endocrinology,The 4th People's Hospital of Shenyang,Liaoning Shenyang 110034,China;2.The Endocrine Institute and the Liaoning Provincial Key Laboratory of Endocrine Diseases,The First Hospital of China Medical University,Liaoning Shenyang 110001,China. |
| |
| Abstract: | Objective:To investigate the effects of IFN-γ on the migration and invasion of the PTC cell line TPC-1,confirmed whether the epithelial-mesenchymal transition (EMT) mechanism was involved in the progression.Methods:The effect of IFN-γ on cell migration and invasion was assessed by Wound-healing and Transwell assay.The expression level of mRNA and protein of EMT makers E-cadherin,N-cadherin and Vimentin was analyzed using RT-PCR and Western blot,and their distributions were detected by immunofluorescence.Results:The Wound-healing and Transwell showed that the migratory and invasive abilities of PTC cell treated with IFN-γ were increaseed (P＜0.05).RT-PCR showed the decreased mRNA level of E-cadherin and the increased mRNA of Vimentin,but there was no obvious increase in mRNA of N-cadherin.Western blot showed that E-cadherin protein expression was decreased,N-cadherin and Vimentin were increased (P＜0.05).These results were further confirmed by immunefluorescence analysis.Conclusion:Our results showed that EMT involved in the malignant progression induced by IFN-γ in PTC cell,imply that inflamemation participated in invasion and metastasis of human papillary thyroid cancer. |
| |
| Keywords: | interferon gamma invasion and metastasis papillary thyroid cancer epithelial mesenchymal transition |
|
| 点击此处可从《现代肿瘤医学》浏览原始摘要信息 |
|
点击此处可从《现代肿瘤医学》下载全文 |
