Investigation of the DNMT3B –579 G>T Promoter Polymorphism in Patients with Colorectal Cancer in an Azerbaijani Population |
| |
| Authors: | Bayram BayramovNuru BayramovHazi AslanovMehraj AbbasovShabnam MammadovaKhagani EynullazadaFarah GahramanovaVugar Yagublu |
| |
| Affiliation: | 1Department of Molecular Genetics and Genomics, Genetic Resources Institute of Azerbaijan National Science Academy, Baku, Azerbaijan.2First Surgical Disease Department, Azerbaijan Medical University, Baku, Azerbaijan.3Department of Surgery, Scientific Center of Surgery, Baku, Azerbaijan.4Department of Genetics, University of Wisconsin-Madison, Madison, USA. |
| |
| Abstract: | Objective: The main aim of the present study was to determine the clinical significance of the DNA methyltransferase 3B (DNMT3B) gene –579 G>T polymorphism in colorectal cancer (CRC) patients. Methods: A total of 140 patients with CRC and 164 healthy individuals were included in the study. According to the manufacturer’s instructions, DNA was isolated from blood, and genotypes were determined on agarose gel by the PCR-RFLP method. Genotype confirmation was performed using Sanger sequencing in randomly selected samples. Results: When comparing the case and control groups, heterozygous GT (OR=0.53; 95% CI=0.32–0.88), under the dominant model (OR=0.53; 95% CI=0.33–0.87), and the mutant T allele (OR=0.71; 95% CI=0.51–0.98) were statistically associated with a reduced risk of CRC. However, when the age, pathological tumor grade and stage, smoking habit, and alcohol consumption were compared, no significant relationship was determined (P>0.05). Furthermore, among males, heterozygous GT was associated with a reduced risk of CRC (OR=0.40; 95% CI=0.19–0.84). Conclusion: Our study highlighted that the –579 G>T polymorphism of the DNMT3B gene plays a protective role against CRC development. |
| |
| Keywords: | DNMT3B Polymorphism DNA Methylation colorectal cancer |
|
| 点击此处可从《Asian Pacific journal of cancer prevention : APJCP》浏览原始摘要信息 |
|
点击此处可从《Asian Pacific journal of cancer prevention : APJCP》下载全文 |
|
