| Midkine对神经母细胞瘤TNB1细胞生长与分化的影响 |
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| 引用本文： | 朱琳,陈潞萍,牟萍.Midkine对神经母细胞瘤TNB1细胞生长与分化的影响[J].中国肿瘤临床,2022,49(3):109-114. |
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| 作者姓名： | 朱琳 陈潞萍 牟萍 |
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| 作者单位： | 1.沈阳医学院基础医学院生物化学与分子生物学教研室 （沈阳市110034） |
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| 基金项目： | 辽宁省“兴辽英才计划”青年拔尖人才项目(编号:XLYC1807116);辽宁省自然科学基金项目(编号:20180550491)资助。 |
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| 摘 要： | 目的:通过对中期因子(midkine,MK)在神经母细胞瘤TNB1细胞中的生物学作用进行研究,揭示该因子在肿瘤细胞生长和分化中相关分子机制.方法:1)利用神经母细胞瘤临床患者数据库,明确MK的表达与神经母细胞瘤患者生存率之间的关系.在体外细胞实验中,利用携带MKshRNA载体的慢病毒体外感染神经母细胞瘤TNB1细胞,分...
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| 关 键 词： | 中期因子 神经母细胞瘤 生长 分化 肾细胞癌下调蛋白1 |
| 收稿时间： | 2021-07-15 |
Effect of midkine on cell growth and differentiation of neuroblastoma TNB1 cells |
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| Lin Zhu,Luping Chen,Ping Mu.Effect of midkine on cell growth and differentiation of neuroblastoma TNB1 cells[J].Chinese Journal of Clinical Oncology,2022,49(3):109-114. |
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| Authors: | Lin Zhu Luping Chen Ping Mu |
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| Affiliation: | 1.Department of Biochemistry and Molecular Biology2.Department of Physiology, Basic Medical School, Shenyang Medical College, Shenyang 110034, China |
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| Abstract: | Objective:To assess biological functions and underlying mechanisms of midkine(MK)in cell growth and differentiation of neuroblastoma TNB1 cells.Methods:First,the correlation between MK expression and survival rate was assessed in patients with neuroblastoma whose data were obtained from a clinical database.Furthermore,cell growth and differentiation ability of TNB1 cells were evaluated after treatment with MK shRNA-containing lentivirus in vitro.Second,the related gene of MK was screened out by analyzing data from the clinical database,and its expression in MK shRNA-treated TNB1 cells was assessed using real-time PCR.Finally,TNB1 cells were infected with MK shRNA-and downregulated in renal carcinoma 1(DRR1)shRNA-containing lentivirus,and the effect of MK-DRR1 axis on cell differentiation and soft agar colony formation was evaluated.Results:Data from the clinical database revealed the negative correlation between MK expression and survival rate of patients with neuroblastoma(P<0.01).Downregulation of MK repressed cell growth and promoted TNB1 cell differentiation.Analysis of the clinical database revealed a negative correlation between MK expression and DRR1 expression(P<0.01).Real-time PCR results confirmed that DRR1 expression was increased in MK shRNA-treated TNB1 cells(P<0.01).Combined treatment with MK shRNA and DRR1 shRNA rescued the effect of MK shRNA on cell differentiation and soft agar colony formation(P<0.01).Conclusions:MK mediated the growth and differentiation of TNB1 cells by regulating DRR1 expression. |
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| Keywords: | midkine(MK) neuroblastoma growth differentiation downregulated in renal carcinoma 1(DRR1) |
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