| TNP-470作用于体外培养GNM细胞系的形态学研究 |
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| 引用本文： | 彭利伟,李金荣.TNP-470作用于体外培养GNM细胞系的形态学研究[J].华西口腔医学杂志,2002,20(3):175-177. |
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| 作者姓名： | 彭利伟 李金荣 |
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| 作者单位： | 450052 郑州大学口腔系(彭利伟现为武汉大学口腔医学院博士研究生),武汉大学口腔医学院(李金荣) |
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| 摘 要： | 目的:观察TNP-470作用于体外培养的人口腔鳞癌颈淋巴结转移癌细胞系GNM后的形态学改变,探讨TNP- 470的作用机理。方法:利用体外培养技术,采用相差显微镜、扫描电镜、透射电镜观察法,观察TNP-470对GNM细胞形态及超微结构的影响。结果:TNP-470(2Lg/ml)作用于GNM细胞48 h,大量细胞变圆、细胞膜皱缩,并出现较多悬浮死亡细胞。作用72 h后,扫描电镜下可见细胞表面的微绒毛数量减少、长度变小、细胞一端有较大的球状突; 透射电镜下可见GNM细胞大量坏死、线粒体和内质网破坏。结论:TNP-470对GNM细胞具有明显杀伤作用;TNP- 470可使GNM细胞线粒体和内质网发生破坏,这可能是其具有直接癌细胞毒作用的机制之一;TNP-470可使GNM 细胞膜表面结构发生改变。
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| 关 键 词： | TNP-470 GNM细胞系 形态学 |
| 收稿时间： | 2002-06-25 |
| 修稿时间： | 2000年8月31日 |
Effect of Angiogenesis Inhibitor (TNP-470) on the Morphology of GNM Cell Line in vitro |
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| Peng Liwei,The.Effect of Angiogenesis Inhibitor (TNP-470) on the Morphology of GNM Cell Line in vitro[J].West China Journal of Stomatology,2002,20(3):175-177. |
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| Authors: | Peng Liwei The |
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| Affiliation: | Peng Liwei The Department ofOral andMaxillofacialSurgery, the FacultyofStomatology, ZhengzhouUniversity Li Jinrong The Stomatological College, Wuhan University |
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| Abstract: | OBJECTIVE: The purpose of this study was to investigate the effect of an angiogenesis inhibitor (TNP-470) on the ultra micro-structural morphological changes of GNM cell line, which was derived from human oral squamous cell carcinomas in vitro. METHODS: The GNM cells were cultured and, the effect of TNP-470 on ultra micro-structural morphological changes of GNM cells was observed under the inverted microscope, the scanning electron microscope (SEM) and the transmission electron microscope (TEM). RESULTS: Numerous round cells, shrinkage of cellular membrane and dead cells were observed 48 hours after 2 micrograms/ml of TNP-470 was added into the GNM cellular suspension. After 72 hours, GNM cells became shortened and, the number of microvilli of the cellular surface was observed under the SEM and TEM. A large number of GNM cells turned into necrosis, accompanying with the destruction of mitochondria and endoplasmic reticula. CONCLUSION: TNP-470 has a strong tumor cytotoxic effect on GNM cells, which may be due to its destructibility on mitochondria and endoplasmic reticula of GNM cells. TNP-470 can alter the surface structure of GNM cell membrane, which suggests that TNP-470 may interrupt the metastasis of GNM cells. |
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| Keywords: | angiogenesis inhibitor GNM cell line cellular morphology |
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