| 卡介苗中PE和PPE家族蛋白B细胞抗原表位多态性研究 |
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| 引用本文： | 李马超,刘海灿,赵秀琴,万康林.卡介苗中PE和PPE家族蛋白B细胞抗原表位多态性研究[J].疾病监测,2016,31(4):282-287. |
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| 作者姓名： | 李马超 刘海灿 赵秀琴 万康林 |
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| 作者单位： | 1.中国疾病预防控制中心传染病预防控制所, 传染病预防控制国家重点实验室, 北京 102206 |
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| 基金项目： | 国家科技重大专项(No.2013ZX10003006-002-001)~~ |
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| 摘 要： | 目的 了解13株卡介苗(bacille calmette-gurin, BCG)基因组中由PE/PPE基因家族编码的B细胞抗原表位分布情况, 分析其对BCG免疫保护力的潜在影响。方法 从国际免疫表位数据库(Immune Epitope Data base, IEDB)中检索结核分枝杆菌B细胞抗原表位, 与结核分枝杆菌参考菌株H37Rv的蛋白质组进行序列比对, 确定PE/PPE基因家族编码B细胞抗原表位序列;并与13株BCG基因组进行序列比对, 提取表位编码序列, 分析其在BCG中的分布与变异。结果 6个PE/PPE基因家族的基因编码28个B细胞抗原表位, 21个B细胞抗原表位在13株BCG中高度保守, 7个B细胞抗原表位在BCG中发生基因变异, 变异表位分别产生于BCG的不同形成期。结论 PE/PPE基因家族所表达的蛋白大多为细菌表面蛋白, PE/PPE蛋白家族中B细胞抗原表位的变化可能对BCG的保护力产生影响, 应继续开展基于B细胞表位的BCG遗传特征研究。
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| 关 键 词： | 卡介苗 抗原表位 PE/PPE基因家族 基因组 |
| 收稿时间： | 2015-12-10 |
Polymorphism of B cell epitopes coded with PE/PPE gene family in BCG |
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| LI Ma-chao;LIU Hai-can;ZHAO Xiu-qin;WAN Kang-lin.Polymorphism of B cell epitopes coded with PE/PPE gene family in BCG[J].Disease Surveillance,2016,31(4):282-287. |
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| Authors: | LI Ma-chao;LIU Hai-can;ZHAO Xiu-qin;WAN Kang-lin |
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| Affiliation: | 1.State Key Laboratory for Communicable Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China |
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| Abstract: | Objective To understand the distribution of B cell epitopes coded with PE/PPE gene family in 13 BCG strains and the influence on BCG immunity. Methods Mycobacterium tuberculosis B cell Epitopes was retrieved from Immune Epitope Data for the alignment with sequence of B cell epitopes of proteome of M. tuberculosis strain H37Rv to determine the PE/PPE gene family coding B cell epitopes. Then the alignment between DNA sequence coding epitopes and genomes of 13 BCG strains was conducted, and the coding sequence in BCG genomes was extracted to analyze its distribution and mutation in BCG. Results Six PE/PPE genes encoded 28 B cell epitopes. 21 B cell epitopes were highly conservative in 13 BCG strains, 7 B cell epitopes showed gene mutation in the BCG genomes. All gene variations in B cell epitopes could occur in different formation periods of BCG. Conclusion PE/PPE proteins expressed by the PE/PPE gene family mostly are bacterial surface protein. The gene mutation of B cell epitope in PE/PPE protein family may affect the BCG protection. It should continue to carry out research on BCG genetic characteristics based on B cell epitopes. |
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| Keywords: | Bacille Calmette-guérin Epitope PE/PPE gene family Genome |
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