| 黑龙江省肠出血性大肠埃希菌遗传基因型别的研究 |
| |
| 引用本文： | 裴迎新,李俐,林玲,孟琳,谭金煜,熊衍文,苏华.黑龙江省肠出血性大肠埃希菌遗传基因型别的研究[J].疾病监测,2011,26(3):172-175. |
| |
| 作者姓名： | 裴迎新 李俐 林玲 孟琳 谭金煜 熊衍文 苏华 |
| |
| 作者单位： | 1.黑龙江省疾病预防控制中心, 黑龙江 哈尔滨 150030; |
| |
| 基金项目： | 黑龙江省自然科学基金(No.LC08C29)~~ |
| |
| 摘 要： | 目的 对肠出血性大肠埃希菌(EHEC)的遗传基因刑别进行不同分子分型方法的对比研究,从分子流行病学角度分析黑龙江省EHEC的检出情况,并对多位点串联重复序列分析(MLVA)分型方法用于 EHEC 的分型效果进行评价.方法 采用毒力基因鉴定、核酸脉冲场凝胶电泳(PFGE)与MLVA 3种方法对70株EHEC进行遗传基因型...
|
| 关 键 词： | 肠出血性大肠埃希菌 脉冲场凝胶电泳 多位点串联重复序列分析 多位点串联重复序列 分型 |
| 收稿时间： | 2010-12-01 |
Genotype of enterohemorrhagic Escherichia coli isolated in Heilongjiang province |
| |
| PEI Ying-xin,LI Li,LIN Ling,MENG Lin,TAN Jin-yu,XIONG Yan-wen,SU Hua.Genotype of enterohemorrhagic Escherichia coli isolated in Heilongjiang province[J].Disease Surveillance,2011,26(3):172-175. |
| |
| Authors: | PEI Ying-xin LI Li LIN Ling MENG Lin TAN Jin-yu XIONG Yan-wen SU Hua |
| |
| Affiliation: | 1.Heilongjiang Provincial Center for Disease Control and Prevention, Harbin 150030, Heilongjiang, China;2.Chinese Field Epidemiology Training Program, Chinese Center for Disease Control and Prevention, Beijing 100050, China |
| |
| Abstract: | Objective To study genotype of enterohemorrhagic Escherichia coli（EHEC） with three different subtyping methods,conduct molecular epidemiological analysis on EHEC detection in Heilongjiang and evaluate MLVA subtying effect on EHEC strains.Methods Seventy isolates of EHEC were analyzed by virulence gene identification,pulsed-field gel electrophoresis（PFGE） and multilocus variable-number tandem repeat analysis（MLVA）.Results Twelve strains contained eaeA gene,seven strains contained hlyA gene,one strain contained stx1 and stx2 gene and 2 strains contained stx2 gene.Thirty five patterns in PFGE clusters were defined as strains that have same pattern by XbaⅠ digestion among 47 isolates,MLVA was performed by comparing the number of tandem repeats at seven loci,where seven loci showed 2 to 5 variations in the 10 isolates of EHEC O157,resulting in 8 MLVA types among the 10 isolates.Conclusion There was horizontal transmission of EHEC in household animal raising.Since null alleles existed in some strains were observed in 6 subtyping sites,more isolates are needed to conduct further study on subtyping effect of MLVA. |
| |
| Keywords: | enterohemorrhagic Escherichia coli pulsed-field gel electrophoresis mltiple-locus variable-number tandemrepeat analysis variable-number tandem repeat subtyping |
| 本文献已被 CNKI 维普 等数据库收录！ |
| 点击此处可从《疾病监测》浏览原始摘要信息 |
|
点击此处可从《疾病监测》下载全文 |
|
