| 2005-2014年上海市O139群霍乱弧菌的分子特征和耐药性研究 |
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| 引用本文： | 屠丽红,张曦,陈洪友,宋元君,陈敏.2005-2014年上海市O139群霍乱弧菌的分子特征和耐药性研究[J].疾病监测,2015,30(3):223-227. |
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| 作者姓名： | 屠丽红 张曦 陈洪友 宋元君 陈敏 |
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| 作者单位： | 1.上海市疾病预防控制中心, 上海 200336 |
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| 摘 要： | 目的 分析2005-2014年上海市O139群霍乱弧菌的分子特征和耐药性。方法 以聚合酶链反应(PCR)方法检测86株O139群霍乱弧菌的霍乱毒素基因(ctxA)、溶血素基因(hlyA)、毒素协调菌毛基因(tcpA)、调控蛋白基因(toxR)和编码霍乱毒素基因的CTX基因组基因(zot、ace)。采用脉冲场凝胶电泳(PFGE)和多位点序列分型(MLST)方法对O139群霍乱弧菌菌株进行分子分型。采用WHO推荐的改良K-B纸片法,对O139群霍乱弧菌菌株进行11种抗菌药物(头孢曲松、强力霉素、诺氟沙星、环丙沙星、复方新诺明、丁胺卡那霉素、四环素、氯霉素、萘啶酸、氨苄西林、庆大霉素)敏感试验。结果 患者来源O139群霍乱弧菌菌株93.2%(41/44)为产毒株,水产来源菌株58.3%(21/36)为产毒株,6株水体来源菌株均为非产毒株。PFGE分析将O139群霍乱弧菌分为35个型别。MLST分析将O139群霍乱弧菌分为9个ST型,其中流行菌型为ST 69型,非产毒株以ST 162型为主。86株O139群霍乱弧菌经药物敏感试验分析显示O139群霍乱弧菌产毒株的耐药情况比非产毒株严重。O139群霍乱弧菌产毒株对强力霉素、复方新诺明、四环素、萘啶酸、氨苄西林、庆大霉素的耐药率高。O139群霍乱弧菌非产毒株对萘啶酸耐药严重。结论 上海市患者和水产来源O139群霍乱弧菌构成以产毒株为主。PFGE分型提示水产为霍乱疫情的主要传染源。O139群霍乱弧菌产毒株高度克隆化,流行菌型为ST 69型,耐药情况严重。
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| 关 键 词： | 霍乱弧菌 毒力基因 脉冲场凝胶电泳 多位点序列分型 耐药性 |
| 收稿时间： | 2014-12-22 |
Molecular characteristics and antibiotic resistance of Vibrio cholerae O139 in Shanghai ,2005-2014 |
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| TU Li-hong,ZHANG Xi,CHEN Hong-you,SONG Yuan-jun,CHEN Min.Molecular characteristics and antibiotic resistance of Vibrio cholerae O139 in Shanghai ,2005-2014[J].Disease Surveillance,2015,30(3):223-227. |
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| Authors: | TU Li-hong ZHANG Xi CHEN Hong-you SONG Yuan-jun CHEN Min |
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| Affiliation: | 1.Department of Microbiology, Shanghai Municipal Center for Disease control and Prevention, Shanghai 200336, China |
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| Abstract: | Objective To describe the molecular characteristics and antibiotic resistance of Vibrio cholerae O139 in Shanghai from 2005 to 2014. Methods PCR was conducted to detect six virulence-related genes of 86 strains of V. cholerae O139, including cholera toxin gene (ctxA), hemolysin gene (hlyA)、toxin-coregulated pilus gene (tcpA), the regulatory protein genes (toxR) and the genes of phage CTX encoding cholera toxin (zot, ace). Pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) were carried out for the molecular subtyping of the strains, and the strains' antibiotic resistance was detected with K-B test. Results Up to 93.2% of V. cholerae O139 strains isolated from patients and 58.3% isolated from aquatic products were toxigenic,while all the V. cholerae O139 strains isolated from environment were non-toxigenic. According to the PFGE pattern, 86 V. cholerae O139 strains belonged to 35 molecular types. According to MLST result, 86 V. cholerae O139 strains belonged to 9 ST types. ST 69 and ST 162 were predominant in Shanghai. The toxigenic V. cholerae O139 strains isolated showed higher resistance to antibiotics compared with the non-toxigenic strains. Conclusion Most V. cholerae O139 strains isolated from patients and aquatic products in Shanghai were toxigenic, PFGE suggested that aquatic products were the main infectious sources of cholera. The toxigenic isolates had similar molecular characteristics and the predominant type was ST 69. The antibiotic resistance of the toxigenic V. cholerae O139 strains was serious. |
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| Keywords: | Vibrio cholerae Virulence-related genes Pulsed-field gel electrophoresis Multilocus sequence typing Antibiotic resistance |
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