| 潜在致病性的弗氏枸橼酸杆菌的筛查 |
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| 引用本文： | 刘丽云,夏胜利,孙晖.潜在致病性的弗氏枸橼酸杆菌的筛查[J].疾病监测,2015,30(2):92-96. |
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| 作者姓名： | 刘丽云 夏胜利 孙晖 |
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| 作者单位： | 1. 中国疾病预防控制中心传染病预防控制所,传染病预防控制国家重点实验室,北京102206
2. 河南省疾病预防控制中心,河南郑州,450016 |
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| 基金项目： | 国家自然科学基金(No.81301401);传染病预防控制国家重点实验室面上项目(No.2011SKLID209)~~ |
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| 摘 要： | 目的 筛查具有潜在致病性的弗氏枸橼酸杆菌。方法 对从河南省睢县分离到的36株弗氏枸橼酸杆菌进行黏附HEp-2细胞的检测,以及与HEp-2细胞(MOI: 100)作用10 h后的乳酸脱氢酶(LDH)释放情况分析。同时比较弗氏枸橼酸杆菌CF74(Citrobacter freundii 74)、CF72(Citrobacter freundii 72)、肠聚集性大肠埃希菌(Enteroaggregative E. coli,EAEC)、肠致病性大肠埃希菌(Enteropathogenic E. coli,EPEC)2348/69和HB101的黏附和细胞毒性差异。结果 16株弗氏枸橼酸杆菌几乎没有黏附性,黏附指数1;18株弗氏枸橼酸杆菌具有中等强度的黏附性,黏附指数100;2株弗氏枸橼酸杆菌CF74和CF65(Citrobacter freundii 65)具有强的黏附性。与HEp-2细胞作用10 h后,除了CF74(24.4%),其余的35株弗氏枸橼酸杆菌具有低的LDH释放量,LDH释放量与阴性对照HB101(5.02%)相似。说明除了CF74,其余的弗氏枸橼酸杆菌不具有细胞毒性。CF74具有和EAEC042一样的聚集性黏附类型,并且 CF74引起的LDH释放率明显高于CF72、2348/69和HB101(P0.01)而低于EAEC17-2。结论 作为潜在的致病菌,CF74具有强的黏附性和细胞毒性。
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| 关 键 词： | 弗氏枸橼酸杆菌 细胞毒性 细胞黏附 乳酸脱氢酶 |
| 收稿时间： | 2014-09-24 |
Screening of potential pathogenic Citrobacter freundii |
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| LIU Li-yun,XIA Sheng-li,SUN Hui.Screening of potential pathogenic Citrobacter freundii[J].Disease Surveillance,2015,30(2):92-96. |
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| Authors: | LIU Li-yun XIA Sheng-li SUN Hui |
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| Affiliation: | LIU Li-yun;XIA Sheng-li;SUN Hui;State Key Laboratory for Communicable Disease Prevention and Control,Institute for Communicable Disease Prevention and Control,Chinese Center for Disease Control and Prevention;Henan Provincial Center for Disease Control and Prevention; |
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| Abstract: | Objective To screen potential pathogenic Citrobacter freundii. Methods The adhesion of C. freundii on HEp-2 cells was tested by using 36 C. freundii strains isolated from Suixian county, Henan province, and lactate dehydrogenase (LDH) released induced by C. freundii was detected after co-cultured with HEp-2 cells for 10 hours. Moreover, the adhesion and cytotoxicity were compared among C. freundii 74, C. freundii 72, enteroaggregative E. coli (EAEC) strain 042 and 17-2, enteropathogenic E. coli (EPEC) strain 2348/69 and E. coli strain HB101. Results Sixteen C. freundii strains showed little adhesion, with indexes 1; 18 C. freundii strains showed intermediate adhesion, with adhesion indexes ranged from 1 to 100; C. freundii 65 and C. freundii 74 showed the strongest adhesion, with adhesion indexes 100. After 10 hours co-culturing with HEp-2 cells, 35 C. freundii strains, except C. freundii 74 (24%), showed low LDH releasing, similar to that induced by positive control HB101 (5.02%). It suggested that all the isolates, except C. freundii 74 were likely to be non-cytotoxic. C. freundii 74 showed an aggregative adherence pattern similar to that of EAEC 042,the LDH releasing induced by C. freundii 74 was higher than C. freundii 72, 2348/69 and HB101 (P0.01), but lower than EAEC17-2. Conclusion As a potential pathogen, C. freundii 74 showed the strongest adhesion and cytotoxicity. |
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| Keywords: | Citrobacter freundii Cytotoxicity Adhesion Lactate dehydrogenase |
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