| 滇西地区室内鼠形动物巴尔通体感染情况调查研究 |
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| 引用本文： | 杜春红,尹家祥,栗冬梅,王秀芳,程晓藕,杨光璨,刘正祥.滇西地区室内鼠形动物巴尔通体感染情况调查研究[J].疾病监测,2016,31(3):220-224. |
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| 作者姓名： | 杜春红 尹家祥 栗冬梅 王秀芳 程晓藕 杨光璨 刘正祥 |
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| 作者单位： | 1.云南省地方病防治所, 云南大理 671000 |
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| 基金项目： | 2014HB093) and Dali College doctoral scientific research foundation (No.KYBS201302) ，国家自然科学基金(No.81060229;81460485)，教育部留学回国人员科研启动基金资助项目(教外司留[2011]1568号)，云南省高层次卫生技术人才培养专项(No.D-201249)，云南省中青年学术和技术带头人后备人才、技术创新人才培养项目(No.2013HB080;2014HB093)，大理学院博士科研启动费项目(No.KYBS201302) |
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| 摘 要： | 目的 了解滇西地区室内鼠形动物巴尔通体感染状况。方法 在滇西地区8个州(市)抽取10个县(市、区)40个自然村,随机抽取800户家庭(每村20户),进行室内捕鼠,采集鼠脾脏标本并提取基因组DNA,运用聚合酶链反应(PCR)扩增巴尔通体枸橼酸合酶(gltA)基因,阳性者进行测序分析。结果 捕获9种421只鼠形动物,采集脏器样本404份,PCR扩增阳性64份,阳性率为15.84%。9种鼠形动物中仅黄胸鼠和褐家鼠检出阳性,阳性率分别为23.05%(62/269)和8.70%(2/23)。10个县(市、区)中6个检出阳性。基因分析表明携带的巴尔通体至少存在6种基因型:B. tribocorum、B. queenslandensis、B. elizabethae、B. rochalimae及2个可能的新型。结论 滇西地区室内鼠形动物感染巴尔通体,且呈现基因多样性特征,部分县(市)阳性率较高,需加强监测和预防控制。
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| 关 键 词： | 巴尔通体 鼠形动物 室内 滇西地区 |
| 收稿时间： | 2015-08-13 |
Investigation of Bartonella infection in small rodents in households in western Yunnan |
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| Affiliation: | 1.Yunnan Institute for Endemic Diseases Control and Prevention, Dali 671000, Yunnan, China2.Public Health School, Dali University, Dali 671000, Yunnan, China3.Institute for Communicable Disease Prevention and Control, Chinese Center for Disease Control and Prevention, Beijing 102206, China |
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| Abstract: | Objective To understand the Bartonella infection status in small rodents in households in western area of Yunnan province. Methods A total of 800 households were randomly selected from 40 natural villages of 10 counties in western Yunnan. Small rodents were captured for 3 consecutive nights in each household. The liver and spleen specimen of small rodents were collected for DNA extraction. The gltA gene of Bartonella was amplified with PCR and gene-sequencing test was conducted for PCR amplification products. Results A total of 421 small rodents, belonging to 9 species, were captured, and 404 specimens were collected and tested with PCR. The Bartonella positive rate was 15.84% (64/404). Of 9 species, the part of specimens from Rattus tanezumi and Rattus norvegicus were positive for Bartonella and the positive rate was 23.05% (62/269) and 8.70% (2/23), respectively. Among 10 counties, the specimens collected from 6 counties were positive. Gene analysis showed that there were 6 gene types of Bartonella infection, which included B. tribocorum, B. queenslandensis, B. elizabethae and B. rochalimae, and 2 possible new gene types were found. Conclusion Bartonella infection was detected in small rodents in households in western Yunnan and the gene diversity was observed. Bartonella infection rate in some counties was high, indicating that the surveillance and control/prevention measures need to be strengthened. |
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| Keywords: | Bartonella Small rodents Household Western Yunnan |
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