| 恶性肿瘤患者UGT1A1基因启动子多态性检测结果分析 |
| |
| 引用本文： | 吴泽刚,李 艳,郑红云,熊 格.恶性肿瘤患者UGT1A1基因启动子多态性检测结果分析[J].现代检验医学杂志,2015,0(3):35-37. |
| |
| 作者姓名： | 吴泽刚 李 艳 郑红云 熊 格 |
| |
| 作者单位： | 武汉大学人民医院检验科,武汉 430060 |
| |
| 摘 要： | 目的 应用基因测序技术检测分析UGT1A1基因启动子多态性特点,并探讨UGT1A1*28和UGT1A1*6基因多态性在武汉地区的分布。方法 收集2013年1月～2014年12月武汉大学人民医院肿瘤内科230例肿瘤患者外周血,通过Sanger测序法测定靶片段的基因序列,分析患者UGT1A1基因启动子区TA盒多态性。结果 研究检测的230例肿瘤患者中,TA6/TA6野生型198例(86.1%),TA6/TA7杂合型29例(12.6%),TA7/TA7变异型3例(1.3%)。结论 武汉地区恶性肿瘤患者UGT1A1基因TA6/TA6野生型最常见。
|
| 关 键 词： | UGT1A1基因 基因测序 多态性 伊立替康 |
Analysis on Gene Polymorphism of UGT1A1 Gene Promoter in Patients with Malignant Tumour |
| |
| WU Ze-gang,LI Yan,ZHENG Hong-yun,XIONG Ge.Analysis on Gene Polymorphism of UGT1A1 Gene Promoter in Patients with Malignant Tumour[J].Journal of Modern Laboratory Medicine,2015,0(3):35-37. |
| |
| Authors: | WU Ze-gang LI Yan ZHENG Hong-yun XIONG Ge |
| |
| Affiliation: | Department of Clinical Laboratory,
Renmin Hospital of Wuhan University,Wuhan 430060,China |
| |
| Abstract: | Objective To detect UGT1A1 gene promoter polymorphism with gene sequence method and analyze the distribution of UGT1A1*28 and UGT1A1*6 gene polymorphism in Wuhan.Methods A total of 230 samples were collected from tumor patient in Medical Oncology Department of Renmin Hospital of Wuhan University from January 2013 to December 2014,then genesequence of the target fragments were detected by Sanger sequencing method and analyzed patient UGT1A1 gene promoter polymorphism TA box.Results In this research examination 230 example tumor patient,198 patients(86.1%)were identified with TA6/TA6 genotype,29 patients(12.6%)were identified with TA6/TA7 genotype,3 patients(1.3%)TA7/TA7 variant.Conclusion TA6/TA6 wild-typewas higher frequence in patients with malignant tumour in Wuhan. |
| |
| Keywords: | |
| 本文献已被 万方数据 等数据库收录！ |
| 点击此处可从《现代检验医学杂志》浏览原始摘要信息 |
|
点击此处可从《现代检验医学杂志》下载全文 |
|
