| 类风湿关节炎患者血清DKK-1，CCL21水平与体液免疫指标的相关性研究 |
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| 引用本文： | 刘素芳,赵 阳,贾 彬,李俊芳.类风湿关节炎患者血清DKK-1，CCL21水平与体液免疫指标的相关性研究[J].现代检验医学杂志,2022,0(3):33-36. |
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| 作者姓名： | 刘素芳 赵 阳 贾 彬 李俊芳 |
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| 作者单位： | （邯郸市中心医院免疫科，河北邯郸 056001） |
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| 摘 要： | 目的 探讨类风湿关节炎（ rheumatoid arthritis ，RA）患者血清 Dickkopf-1（DKK-1），趋化因子（ chemokine， CCL）21水平与体液免疫指标的相关性。方法 选择 2018年 3月～ 2020年 6月邯郸市中心医院免疫科收治的 120例 RA患者（ RA组）和 117例体检志愿者（对照组），根据 28个关节疾病活动度（ disease activity score 28，DAS28）评分将 RA组分为低度组（ 2.6～ 3.2分，38例）、中度组（ 3.3～ 5.1分，50例）和重度组（＞ 5.1分，32例）。检测血清 DKK-1，CCL21，免疫球蛋白 A（immunoglobulin A， IgA）、免疫球蛋白 G（IgG）、免疫球蛋白 M（IgM）、免疫球蛋白 E（IgE）、补体 C3和补体 C4水平。分析 DKK-1，CCL21与 IgG，IgA，IgM，IgE，补体 C3，补体 C4之间相关性。结果 RA组血清 DKK-1（72.14±15.26 pg/ml），CCL21（285.16±32.16 pg/ml），IgG（16.35±4.56 g/L），IgA（3.65±0.52 g/L）和 IgM（1.95±0.42 g/L）水平高于对照组（ 43.26±7.46 pg/ml，170.06±20.41pg/ml，10.35±1.65g/L，2.02±0.24g/ L，1.42±0.39g/L），差异均有统计学意义（ t=10.061～ 32.803，均 P＜ 0.05），补体 C3（1.05±0.16 g/L）和补体 C4（0.15±0.07 g/L）水平低于对照组（ 1.39±0.25g/L，0.36±0.11g/L），差异均有统计学意义（ t=12.502，17.579，均 P＜ 0.05）。重度组血清 DKK-19（83.26±3.09 pg/ml），CCL21（310.06±6.45 pg/ml），IgG(19.84±0.68 g/L），IgA（4.14±0.03 g/ L）和 IgM（2.28±0.06 g/L）水平高于中度组（ 74.90±6.59 pg/ml，289.75±12.54 pg/ml，17.40±2.11 g/L,3.71±0.26 g/ L,2.03±0.16 g/L）和轻度组（59.15±2.34 pg/ml, 258.15±4.06 pg/ml, 12.03±0.21 g/L, 3.16±0.02 g/L, 1.57±0.03 g/L），差异均有统计学意义（ t=6.322～ 162.999, 均 P＜ 0.05）；补体 C3和补体 C4水平低于中度组 (1.18±0.02 g/L vs 1.07±0.05 g/L，0.20±0.01 g/L vs 0.16±0.04 g/L)和轻度组 (0.91±0.02 g/L，0.09±0.01 g/L), 差异均有统计学意义（t= 5.536～ 45.847，均 P＜ 0.05）。中度组血清 DKK-1，CCL21，IgG，IgA和 IgM水平高于轻度组（ t=12.993～ 17.468，均 P＜ 0.05）；补体 C3和补体 C4水平低于轻度组（ t=18.607，10.527，均 P＜ 0.05）,差异均有统计学意义。 RA患者血清 DKK-1和 CCL21水平与 IgG，IgA和 IgM呈正相关（ r=0.742～ 0.805，均 P＜ 0.05），与补体 C3和补体 C4水平呈负相关（ r= -0.736～ -0.744，均 P＜ 0.05），各组之间的 IgE差异均无统计学意义 (F=0.869，P＞ 0.05)，DKK-1和 CCL21与 IgE也无相关性 (r=0.168，0.203，均 P＞ 0.05)。结论 RA患者血清 DKK-1和 CCL21水平升高与 RA疾病活动程度以及 IgG，IgA和 IgM增高与补体 C3和 C4消耗有关。
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| 关 键 词： | 类风湿关节炎 Dickkopf-1 趋化因子 体液免疫 |
Study on the Correlation between Serum DKK-1, CCL21 and Humoral Immune Indexes in Patients with Rheumatoid Arthritis |
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| LIU Su-fang,ZHAO Yang,JIA Bin,LI Jun-fang.Study on the Correlation between Serum DKK-1, CCL21 and Humoral Immune Indexes in Patients with Rheumatoid Arthritis[J].Journal of Modern Laboratory Medicine,2022,0(3):33-36. |
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| Authors: | LIU Su-fang ZHAO Yang JIA Bin LI Jun-fang |
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| Affiliation: | (Department of Immunology ,the Central Hospital of Handan City , Hebei Handan 056001,China) |
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| Abstract: | Objective To investigate the correlation between serum Dickkopf-1 (DKK-1), chemokine (CCL21) and humoral immune indexes in patients with rheumatoid arthritis (RA). Methods A total of 120 RA patients (RA group) and 117 physical examination volunteers (control group) admitted to Department of Immunology,the Central Hospital of Handan City from March 2018 to June 2020 were selected. According to DAS28 scores, the RA group was divided into low-grade group (2.6~3.2 points, 38 cases) and moderate group (3.3 ～ 5.1 points, 50 cases) and severe group ( ＞ 5.1 points, 32 cases).Serum levels of DKK-1, CCL21, immunoglobulin A (IgA), immunoglobulin G (IgG), immunoglobulin M (IgM), immunoglobulin E (IgE), complement C3 and complement C4 were detected.The correlation between DKK-1, CCL21 and IgG, IgA, IgM, IgE, complement C3 and complement C4 was analyzed. Results The serum levels of DKK-1(72.14±15.26 pg/ml)，CCL21(285.16±32.16 pg/ml)， IgG(16.35±4.56 g/L)，IgA(3.65±0.52 g/L) and IgM(1.95±0.42 g/L ) in RA group were higher than those in control group（43.26±7.46 pg/ml, 170.06±20.41pg/ml, 10.35±1.65g/L, 2.02±0.24g/L，1.42±0.39g/L）, the differences were statistically significant (t=10.061~32.803, all P ＜ 0.05), and the levels of complement C3(1.05±0.16 g/L) and complement C4(0.15±0.07 g/L) were lower than those in control group （1.39±0.25g/L，0.36±0.11g/L）, the differences were statistically significant (t=12.502，17.579，all P ＜ 0.05).The serum levels of DKK-19（83.26±3.09 pg/ml）, CCL21（310.06±6.45 pg/ ml）, IgG(19.84±0.68 g/L）, IgA（4.14±0.03 g/L）and IgM（2.28±0.06 g/L） in severe group were higher than those in moderate group（74.90±6.59 pg/ml，289.75±12.54 pg/ml，17.40±2.11 g/L, 3.71±0.26 g/L, 2.03±0.16 g/L） and mild group（59.15±2.34 pg/ml, 258.15±4.06 pg/ml, 12.03±0.21 g/L,3.16±0.02 g/L,1.57±0.03 g/L）, the differences were statistically significant（t=6.322 ～ 162.999, all P ＜ 0.05）, and the levels of complement C3 and complement C4 in severe group were lower than those in moderate group(1.18±0.02 g/L vs 1.07±0.05 g/L，0.20±0.01 g/L vs 0.16±0.04 g/L) and mild group(0.91±0.02 g/L，0.09±0.01 g/L) ，the differences were statistically significant（t= 5.536 ～ 45.847, all P ＜ 0.05）. The serum levels of DKK-1, CCL21, IgG, IgA and IgM in moderate group were higher than those in mild group (t=12.993 ～ 17.468, all P ＜ 0.05), and the levels of complement C3 and complement C4 were lower than those in mild group (t=18.607, 10.527, all P ＜ 0.05) ，the differences were statistically significant，respectively.Serum DKK-1 and CCL21 levels in RA patients were positively correlated with IgG, IgA and IgM (r=0.742 ～ 0.805, all P ＜ 0.05), and negatively correlated with complement C3 and complement C4 levels (r=-0.736 ～ -0.744, all P ＜ 0.05). There was no significant difference in IgE between groups (F=0.869, P ＞ 0.05), DKK-1 and CCL21 were not correlated with IgE (r =0.168, 0.203 , all P ＞ 0.05).Conclusion Serum DKK-1 and CCL21 levels were increased in RA patients, and the increase of DKK-1 and CCL21 levels were related to the activity of RA disease, the increase of IgG, IgA, IgM, and the depletion of complement C3 and C4. |
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