| 支气管扩张伴支气管哮喘患者血清中microRNA-146水平表达与肺功能相关性分析 |
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| 引用本文： | 韩雪娇,刘红红,邢宁宁,李爱军.支气管扩张伴支气管哮喘患者血清中microRNA-146水平表达与肺功能相关性分析[J].现代检验医学杂志,2022,0(2):100-104. |
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| 作者姓名： | 韩雪娇 刘红红 邢宁宁 李爱军 |
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| 作者单位： | （邢台市第三医院呼吸科，河北邢台 054000） |
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| 摘 要： | 目的 探讨微小核糖核酸（microRNA，miRNA/miR）-146 在支气管扩张伴支气管哮喘患者血清中的表达及对肺功能的影响。方法 选取邢台市第三医院2017 年1 月～ 2020 年8 月收治的156 例支气管扩张伴支气管哮喘患者作为研究对象，并以同期150 例健康体检者作为对照组。采用实时定量聚合酶链式反应（real-time quantitative polymerase chain reaction，RQ-PCR）检测miR-146 表达水平，采用酶联免疫吸附法测定血清炎性因子 白细胞介素（interleukin）-4, 干扰素（interferonγ，IFN-γ）] 水平，并采用肺功能仪检测其肺功能指标，采用Pearson 相关性分析miR-146 表达水平与肺功能之间的关系。结果 对照组与观察组的肺功能指标分别为用力肺活量（forced vital capacity, FVC）（3.34±0.92 L vs 2.68±0.84 L），一秒用力呼气容积（forced expiratory volumein in one second, FEV1）（2.03±0.26 L vs 1.65±0.23 L）, FEV1/FVC（82.67%±7.14% vs 65.03%±6.96%）、最大呼气中期流量（maximal mid-expiratory flow, MMEF75 /25）（1.28±0.23L/s vs0.57±0.12L/s）和最大呼气峰值流速（peak expiratory flow, PEF）（545.28±15.89 L/min vs 140.57±13.65 L/min ）。两组相比，观察组的肺功能指标FVC，FEV1，FEV1 /FVC，MMEF75 /25 和PEF 均明显降低，差异有统计学意义（t=6.558 ～ 239.284，均P ＜ 0.01)。观察组患者血清miR-146a（2.51±0.68），miR-146b RNA（1.68±0.49），IL-4（325.41±32.78ng/L） 和INF-γ（232.12±18.48ng/L）表达水平显著高于对照组（0.98±0.41，1.01±0.36，228.69±26.15ng/L，202.25±17.36ng/L），经t 检验，差异具有统计学意义（t=13.667 ～ 28.587，均P ＜ 0.001）。Pearson 相关性分析结果显示支气管扩张伴支气管哮喘患者血清miRNA-146a, miRNA-146bmRNA 表达水平与FVC, FEV1, FEV1/FVC, MMEF75/25 和PEF 等肺功能指标存在显著负相关性（r= -0.687 ～ -0.458, 均P ＜ 0.05），同时与炎症因子IL-4, INF-γ 表达水平存在显著正相关性（r=0.698 ～ 0.745, 均P ＜ 0.001）。结论 miR-146 在支气管扩张伴支气管哮喘血清中呈显著高表达，且miR-146 表达水平与肺功能指标存在明显负相关性，对于肺功能评估具有一定价值。
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| 关 键 词： | 微小核糖核酸 微小核糖核酸-146 支气管扩张伴支气管哮喘 肺功能 |
Correlation Analysis between the Expression Level of micro RNA-146 in Serum and Lung Function in Patients with Bronchiectasis and Bronchial Asthma |
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| HAN Xue-jiao,LIU Hong-hong,XING Ning-ning,LI Ai-jun.Correlation Analysis between the Expression Level of micro RNA-146 in Serum and Lung Function in Patients with Bronchiectasis and Bronchial Asthma[J].Journal of Modern Laboratory Medicine,2022,0(2):100-104. |
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| Authors: | HAN Xue-jiao LIU Hong-hong XING Ning-ning LI Ai-jun |
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| Affiliation: | （Department of Pneumology, the Third Hospital of Xingtai, Hebei Xingtai 054000,China） |
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| Abstract: | Objective To investigate the expression of microRNA (miRNA/miR)-146 in the serum of patients with bronchiectasis and bronchial asthma and its influence on lung function. Methods 156 patients with bronchiectasis and bronchial asthma admitted in the Third Hospital of Xingtai from January 2017 to August 2020 were the subjects of the study, and 150 healthy subjects during the same period were used as the control group. The real-time quantitative polymerase chain reaction method was used to detect the expression level of miR-146, the enzyme-linked immunosorbent assay was used to determine the level of serum inflammatory factors and the lung function was used to test their lung function indicators.Pearson correlation was used to analyze the relationship between miR-146 expression level and lung function. Results The lung function indexes of the control group and the observation group were FVC (3.34±0.92L vs 2.68±0.84L), FEV1 (2.03±0.26L vs 1.65±0.23L), FEV1 /FVC (82.67±7.14% vs 65.03±6.96%), MMEF75/25 (1.28±0.23L/s vs 0.57±0.12L/s) and PEF (545.28±15.89L/min vs 140.57±13.65L/min), respectively. Compared with the two groups, the lung function indexes FVC, FEV1, FEV1 /FVC, MMEF75/25 and PEF in the observation group were significantly decreased ，the differences were statistically significant (t=6.558~239.284, all P<0.01). Serum miR-146a (2.51±0.68), miR-146b mRNA (1.68±0.49), the expression levels of IL-4 (325.41±32.78ng/L) and INF-γ (232.12±18.48ng/L) were significantly higher than those of the control group (0.98±0.41, 1.01±0.36, 228.69±26.15ng/L, 202.25±17.36ng/L), the differences were statistically significant (t= 13.667~28.587, all P < 0.001). Pearson correlation analysis showed that the expression levels of miRNA-146a and miRNA -146b in patients with bronchiectasis and bronchial asthma were significantly correlated with FVC,FEV1, FEV1 /FVC and MMEF75/25. There was a significant negative correlation between PEF and other lung function indexes (r =-0.687~0.458, P<0.05), and a significant positive correlation between PEF and the expression levels of inflammatory cytokines IL-4 and INF-γ (r=0.698~0.745, all P<0.001). Conclusion MiR-146 was highly expressed in the serum of bronchiectasis with bronchial asthma, and the expression level of miR-146 was significantly negatively correlated with lung function, which has certain value for lung function assessment. |
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