| HIV/TB并发感染患者外周血 T淋巴细胞表达及与 HIV RNA载量的相关性研究 |
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| 引用本文： | 李 雪,丁 艳,黄 玲,严加来.HIV/TB并发感染患者外周血 T淋巴细胞表达及与 HIV RNA载量的相关性研究[J].现代检验医学杂志,2022,0(1):88-91. |
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| 作者姓名： | 李 雪 丁 艳 黄 玲 严加来 |
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| 作者单位： | （1. 南京中医药大学附属南京医院& 南京市第二医院检验科，南京市公共卫生医疗中心，南京 210003；2. 安徽医学高等专科学校医学技术系，合肥 230601） |
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| 摘 要： | 目的 探讨人类免疫缺陷病毒 /结核病（ human immunide.ciency virus/tuberculosis，HIV/TB）并发感染患者的外周血 T淋巴细胞亚群的表达及其与 HIV RNA载量的相关性。方法 选取 2018年 6月～ 2020年 6月在南京市公共卫生医疗中心就诊的 HIV/TB并发感染患者 38例作为 HIV/TB组，另选取单纯人类免疫缺陷病毒 /获得性免疫缺陷综合征（human immunode.ciency virus/acquired immune de.ciency syndrome，HIV/AIDS）39例作为 HIV组，单纯 TB患者 35例作为 TB组，体检健康者 35例作为健康对照组。流式细胞术检测各组 CD3+，CD4+，CD8+ T细胞计数和 CD4+/CD8+比值；荧光定量 PCR检测 HIV RNA载量。采用 SPSS17.0统计学软件进行统计分析，并进行 Spearman相关系数分析。结果 CD3+，CD4+和 CD8+ T细胞数， CD4+/CD8+比值在各组中的差异均具有统计学意义（ H=40.758，104.279，9.637和 101.770，均 P＜ 0.05），组间两两比较结果表明，与健康对照组相比， TB组 CD3+和 CD8+T细胞数明显降低，差异均有统计学意义（ Z=-2.520，-2.972，P=0.012，0.003）；HIV组和 HIV/TB组 CD3+，CD4+ T细胞数以及 CD4+/CD8+比值均明显降低（ Z=-7.391~-4.325，均 P=0.000）。与 TB组相比， HIV组 CD3+， CD4+ T细胞数和 CD4+/CD8+比值均明显降低，差异均有统计学意义（ Z=-2.138，-7.032和 -7.380，P=0.032，0000和 0.003），但 CD8+ T细胞数明显升高（Z=-2.463，P=0.014）；而 HIV/TB组 CD3+和 CD4+ T细胞数以及 CD4+/CD8+比值均明显降低，差异均有统计学意义（Z=-3.865，-6.907和 -6.759，均 P=0.000）。与 HIV组相比， HIV/TB组的各项流式指标及 HIV RNA载量差异均无统计学意义（均 P >0.05）。HIV RNA载量与 CD3+，CD4+，CD8+ T细胞数以及 CD4+/CD8+比值均呈显著负相关（ r分别为 -0.327，-0.370，-0.296和 -0.327，均 P <0.05）。结论 HIV/TB并发感染患者外周血 CD3+，CD4+ T细胞数和 CD4+/CD8+比值均明显降低。无论有无并发 TB感染，HIV感染者的细胞免疫功能均随着病毒载量的升高而显著降低。
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| 关 键 词： | 人类免疫缺陷病毒 结核 并发感染 T淋巴细胞 艾滋病病毒载量 |
Study on the Expression of T Lymphocytes in Peripheral Blood and Its Correlation with HIV RNA in HIV/TB Co-Infected Patients |
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| LI Xue,DING Yan,HUANG Ling,YAN Jia-lai.Study on the Expression of T Lymphocytes in Peripheral Blood and Its Correlation with HIV RNA in HIV/TB Co-Infected Patients[J].Journal of Modern Laboratory Medicine,2022,0(1):88-91. |
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| Authors: | LI Xue DING Yan HUANG Ling YAN Jia-lai |
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| Affiliation: | （1. Department of Clinical Laboratory, Affiliated Nanjing Hospital of Nanjing University of Traditional ChineseMedicine &Nanjing Second Hospital, Nanjing Public Health Medical Center, Nanjing 210003, China; 2. Department ofLaboratory Medicine, Anhui Medical College, Hefei 230601, China） |
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| Abstract: | Objective To explore the expression of T lymphocytes in peripheral blood and its correlation with HIV RNA load inHIV/TB co-infected patients. Methods Totally 38 HIV/TB co-infected patients treated in Nanjing Public Health Medical Centerfrom June 2018 to June 2020 were selected as the HIV/TB group. Another 39 HIV/AIDS patients, 35 TB patients and 35 cases ofhealthy people in the same period were randomly selected as control groups. Flow cytometry was used to detect CD3+, CD4+,CD8+ T cell count and CD4+/CD8+ ratio in each group. Fluorescent PCR quantitative method was used to detect the HIV RNAload. Statistical analysis was carried out by using SPSS17.0 statistical software, and Spearman correlation coefficient wasanalyzed. Results There were significant differences in CD3+, CD4+ and CD8+ T cell count and the ratio of CD4+/CD8+ in eachgroup (H = 40.758,104.279,9.637 and 101.770, all P < 0.05). Further pairwise comparison showed that CD3+ and CD8+T cellcount in the TB group were significantly decreased than that in the healthy control group, the differences were statisticallysignificant (Z=-2.520, -2.972, P=0.012，0.003). CD3+, CD4+ T cell count and the ratio of CD4+/CD8+ were significantlydecreased in HIV group and HIV/TB group, the differences were statistically significant (Z=-7.391~ -4.325, all P=0.000).Compared with TB group, CD3+, CD4+ T cell count and the ratio of CD4+/CD8+ were significantly decreased, the differences werestatistically significant(Z= -2.138, -7.032, -7.380, P=0.032，0.000，0.003), but CD8+ T cell count was significantly increased inHIV group (Z= -2.463, P=0.014). However, CD3+ and CD4+ T cell count and the ratio of CD4+/CD8+ decreased significantly inHIV/TB group, the differences were statistically significant(Z=-3.865, -6.907, -6.759, all P=0.000). Compared with the HIVgroup, there was no statistically significant differences in flow parameters and HIV RNA load in HIV/TB group (all P>0.05).CD3+, CD4+, CD8+ cell count and the ratio of CD4+/CD8+ were negatively correlated with HIV RNA (r= -0.327, -0.370, -0.296and -0.327, all P< 0.05). Conclusion CD3+ and CD4+ T cell count and the ratio of CD4+/CD8+ were significantly decreased inHIV/TB co-infected patients. The cellular immune function of HIV patients was significantly decreased with the increase of HIVRNA copies, whether or not they were co-infected with TB. |
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