| 视网膜母细胞瘤组织中miR-142的表达及对细胞增殖和侵袭力的影响 |
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| 引用本文： | 罗 钢,蔡丽英,呙 明.视网膜母细胞瘤组织中miR-142的表达及对细胞增殖和侵袭力的影响[J].现代检验医学杂志,2022,0(3):144-148. |
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| 作者姓名： | 罗 钢 蔡丽英 呙 明 |
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| 作者单位： | （1. 鄂东医疗集团黄石市中心医院湖北理工学院附属医院，湖北黄石 435000；2. 荆州市中心医院，湖北荆州 434000） |
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| 摘 要： | 目的 探讨微小核糖核酸（ micro RNA, miR）-142在视网膜母细胞瘤（ retinoblastoma，RB）组织中的表达，以及对细胞增殖和侵袭力的影响。方法 选取 2013年 6月～ 2019年 6月在黄石市中心医院接受眼球摘除手术治疗的 RB患儿 79例（79眼），培养人 RB细胞株 Y79并分为 miR-142模拟物组、阴性对照组和空白组，分别转染 miR-142模拟物、阴性对照序列和不作处理， RT-qPCR检测 RB和癌旁组织、细胞中 miR-142表达，MTT法检测细胞增殖活性， Transwell法检测细胞迁移和侵袭力。结果 miR-142在 RB组织中表达量（ 0.34±0.12）低于癌旁组织（ 0.99±0.16），差异有统计学意义（ t=29.102，P<0.01）；miR-142表达量在不同分化程度、是否神经浸润和淋巴结转移中差异均有统计学意义（ t=2.991～ 3.495，均 P<0.05）；miR-142模拟物组细胞中 miR-142表达量（ 2.42±0.11）高于阴性对照组和空白组（1.02±0.09，1.01±0.10），差异有统计学意义（ F=398.036，P<0.01）；miR-142模拟物组 24 h，48 h，72 h和 96 h时吸光度 A值、迁移细胞数和侵袭细胞数均低于阴性对照组和空白组（F=5.519, 8.666, 12.926, 21.572, 28.394, 27.982, 均 P<0.05）。结论 RB患者组织中 miR-142表达量降低，且与分化程度、神经浸润和淋巴结转移有关。上调 miR-142表达可抑制 Y79细胞增殖、迁移和侵袭能力。
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| 关 键 词： | 视网膜母细胞瘤 微小核糖核酸-142 细胞增殖 细胞侵袭 |
Expression of miR-142 in Retinoblastoma Tissues and Influences on Cell Proliferation and Invasiveness |
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| LUO Gang,CAI Li-ying,GUO Ming.Expression of miR-142 in Retinoblastoma Tissues and Influences on Cell Proliferation and Invasiveness[J].Journal of Modern Laboratory Medicine,2022,0(3):144-148. |
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| Authors: | LUO Gang CAI Li-ying GUO Ming |
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| Affiliation: | (1. Huangshi Central Hospital of Edong Medical Group /Affiliated Hospital of Hubei Institute of Technology, Hubei Huangshi 435000, China; 2. Jingzhou Central Hospital, Hubei Jingzhou 434000, China) |
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| Abstract: | Objective To investigate the expression of miR-142 in retinoblastoma tissues, and explore its effect on cell proliferation and invasion. Methods A total of 79 children (79 eyes) with RB who underwent eyeball enucleation surgery in Huangshi Central Hospital were selected from June 2013 to June 2019. The human RB cell line Y79 was cultured and divided into miR-142 mimic group, negative control group and blank group, respectively transfected with miR-142 mimic, negative control sequence and no treatment. The expressions of miR-142 in RB and adjacent tissues and cells were detected by using RT-qPCR. The cell proliferation activity was detected with MTT method.The cell migration and invasion were detected with Transwell method. Results The expression level of miR-142 in PB tissues was 0.34±0.12, which was lower than 0.99±0.16 in adjacent tissues，the difference was statistically significant (t=29.102, P<0.01). The differences of the expression level of miR-142 between different differentiation, whether nerve infiltration and lymph node metastasis were statistically significantly, the differences were statistically significant (t=2.991 ~ 3.495, all P<0.05). The expression level of miR-142 in the miR-142 mimic group（2.42±0.11） was higher than that in the negative control group and the blank group（1.02±0.09, 1.01±0.10）, the difference was statistically significant(F=398.036, P<0.05). The absorbance A values at 24 h, 48 h, 72 h and 96 h, the numbers of migrating cells and invadingcells in the miR-142 mimic group were lower than those in the negative control group and the blank group (F=5.519, 8.666, 12.926, 21.572, 28.394, 27.982, all P<0.05). Conclusion The expression level of miR-142 in the RB tissues was reduced, and it was related to the degree of differentiation, nerve infiltration and lymph node metastasis. Up-regulating the expression of miR-142 can inhibit the proliferation, migration and invasion of Y79 cells. |
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