| 克罗恩病患者血清总 25羟维生素 D及细胞因子水平表达与疾病活动指数的相关性研究 |
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| 引用本文： | 杨莉丽,江丹玲,陈文科,关幸求,邹 兵.克罗恩病患者血清总 25羟维生素 D及细胞因子水平表达与疾病活动指数的相关性研究[J].现代检验医学杂志,2022,0(1):195-198. |
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| 作者姓名： | 杨莉丽 江丹玲 陈文科 关幸求 邹 兵 |
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| 作者单位： | （北京大学深圳医院，广东深圳 518036） |
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| 摘 要： | 目的 探讨克罗恩病患者血清总 25羟基维生素 D、细胞因子白介素 6（IL-6）、白介素 8（IL-8）和肿瘤坏死因子α（ TNF-α）在克罗恩病不同时期的表达水平以及其与疾病活动指数（ CDAI）的相关性。方法 选取 2018年 1月～ 2020年 12月在北京大学深圳医院就诊的克罗恩病患者共 100例纳入研究。收集患者的空腹静脉血，检测总 25羟基维生素 D，IL-6，IL-8和 TNF-α水平；采用 CDAI评估患者疾病活动情况，并根据 CDAI评分将患者分为四组，缓解期 21例，轻度活动期 32例，中度活动期 29例和重度活动期 18例。比较 CDAI评分不同分期患者的血清总 25-羟基维生素 D，IL-6，IL-8和 TNF-α的表达水平。并进一步分析比较患者 CDAI与血清总 25-羟基维生素 D水平及细胞因子 IL-6，IL-8及 TNF-α表达水平之间的相关性。结果 四组患者血清总 25-羟基维生素 D及细胞因子 IL-6，IL-8和 TNF-α的差异均存在统计学意义（F=34.281～ 128.53，均 P<0.05）。缓解期患者血清总 25-羟基维生素 D（23.49±3.46μg/
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| 关 键 词： | 克罗恩病 疾病活动指数 总 25-羟基维生素 D 细胞因子 |
Correlation Study on Serum Total 25-Hydroxyvitamin D and Cytokine Levels with Disease Activity Index in Patients withCrohn’s Disease |
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| YANG Li-li,JIANG Dan-ling,CHEN Wen-ke,GUAN Xing-qiu,ZOU Bing.Correlation Study on Serum Total 25-Hydroxyvitamin D and Cytokine Levels with Disease Activity Index in Patients withCrohn’s Disease[J].Journal of Modern Laboratory Medicine,2022,0(1):195-198. |
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| Authors: | YANG Li-li JIANG Dan-ling CHEN Wen-ke GUAN Xing-qiu ZOU Bing |
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| Affiliation: | （Peking University Shenzhen Hospital , Guangdong Shenzhen 518036,China） |
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| Abstract: | Objective To investigate the expression levels of serum total 25-hydroxyvitamin D, cytokines interleukin-6 (IL-6),interleukin-8 (IL-8) and tumor necrosis factor α (TNF-α) in patients with Crohn’s disease at different stages of Crohn’s diseaseand their correlation with Crohn’s disease activity index (CDAI). Methods A total of 100 patients with Crohn’s disease whowere treated at Peking University Shenzhen Hospital from January 2018 to December 2020 were included in the study. Fastingvenous blood of the patients was collected, and the levels of total 25-hydroxyvitamin D, IL-6, IL-8 and TNF-α were determined.Crohn’s disease activity index (CDAI) was used to evaluate patients’ disease activity. According to CDAI score, the patients weredivided into four groups, 21 cases in remission stage, 32 cases in mild active stage, 29 cases in moderate active stage, and 18 casesin severe active stage. The expression levels of serum total 25-hydroxyvitamin D, IL-6, IL-8 and TNF-α in patients with differentCDAI scores were compared. The correlation between CDAI and serum total 25-hydroxyvitamin D levels and the expressionlevels of cytokines IL-6, IL-8 and TNF-α was further analyzed and compared. Results There were significant statisticaldifferences in serum total 25-hydroxyvitamin D and cytokines IL-6, IL-8 and TNF-α among 4 groups (F=34.281~128.53,P<0.05). The serum levels of total 25-hydroxyvitamin D (23.49±3.46μg/L), IL-8 (86.39±11.23ng/L) and TNF-α(24.05±2.12ng/L) in patients in remission stage were significantly higher than those in the other three groups (mild activity stage:19.47±2.83μg/L, 70.48±9.48ng/L and 20.59±3.22ng/L. Moderately active stage: 14.30±2.64μg/L, 65.38±10.91ng/Land 17.48±4.12ng/L. Severe active stage: 11.39±3.93μg/L and 46.58±12.32ng/L, 13.44±3.98ng/L), but the level of IL-6(4.37±0.78ng/L) was significantly lower than that in the other three groups (6.48±1.12ng/L in mild active stage,65.38±10.91ng/L in moderate active stage, 46.58±12.32ng/L in severe active stage), the differences were statistically significant(all P<0.05). Spearman correlation analysis showed that CDAI was negatively correlated with serum total 25-hydroxyvitamin D(R=-0.691, P<0.05) and positively correlated with cytokine IL-6 (r=0.889, P<0.05) in Crohn’s disease patients. And it wassignificantly negatively correlated with IL-8 and TNF-α (r=-0.675, -0.658, all P<0.05). Conclusion There were significantdifferences in total 25-hydroxyvitamin D and cytokine levels among patients with Crohn’s disease at different disease activitystages. Serum IL-6 levels were positively correlated with disease activity, while total 25-hydroxyvitamin D, IL-8 and TNF-αlevels were negatively correlated with disease activity. |
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