| 外源性信号通路JAK/STAT在ALA-光动力诱导人SCC细胞凋亡中的作用 |
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| 引用本文： | 乔丽,李强,蔡宏,田蓉,杨志勇,梅竹松,李菲.外源性信号通路JAK/STAT在ALA-光动力诱导人SCC细胞凋亡中的作用[J].中国麻风皮肤病杂志,2020,36(8):473-478. |
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| 作者姓名： | 乔丽 李强 蔡宏 田蓉 杨志勇 梅竹松 李菲 |
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| 作者单位： | 1空军特色医学中心皮肤科，北京，100142；2空军特色医学中心临床医学实验室，北京，100142 |
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| 摘 要： | 目的：明确外源性凋亡机制(JAK/STAT信号通路)在5-ALA-PDT诱导人鳞状细胞癌细胞(SCC)凋亡中的作用。方法：体外培养鳞状细胞癌细胞系A431和COLO-16，分为空白对照组、转染阴性对照组、光动力组、转染阴性+光动力组、STAT3高表达载体组、STAT3-siRNA组、STAT3高表达载体+光动力组，STAT3-siRNA组+光动力组。CCK-8，流式细胞术（FCM）法分别检测各组细胞的存活率和凋亡率，qRT-PCR和Western Blot检测STAT3及其下游基因Bax和Bcl-2的mRNA和蛋白水平。结果：5-ALA-PDT组A431和COLO-16凋亡率为24%和22%高于STAT3高表达载体+光动力组(11%和10.5%)，低于 STAT3-siRNA+光动力组（36%和39%）；5-ALA-PDT组STAT3和Bcl-2 mRNA水平和蛋白水平低于高表达载体+光动力组，高于STAT3-siRNA组。5-ALA-PDT组Bax mRNA和蛋白的水平高于高表达载体+光动力组，低于STAT3-siRNA组。结论：外源性凋亡通路JAK-STAT3可能参与5-ALA-PDT诱导的上皮鳞癌细胞的凋亡过程。
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| 关 键 词： | 光动力学疗法 5-氨基乙酰丙酸 皮肤鳞状细胞癌 JAK/STAT3 |
Effects of exogenous signal transduction pathway JAK/STAT on apoptosis of human SCC cells induced by ALA-PDT |
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| QIAO Li,LI Qiang,CAI Hong,TIAN Rong,YANG Zhiyong,MEI Zhusong,LI Fei.Effects of exogenous signal transduction pathway JAK/STAT on apoptosis of human SCC cells induced by ALA-PDT[J].China Journal of Leprosy and Skin Diseases,2020,36(8):473-478. |
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| Authors: | QIAO Li LI Qiang CAI Hong TIAN Rong YANG Zhiyong MEI Zhusong LI Fei |
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| Affiliation: | 1 Department of Dermatology, Air Force Characteristic Medical Center, Beijing 100142, China; 2 Clinical Medicine Laboratory, Air Force Characteristic Medical Center, Beijing 100142, China |
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| Abstract: | Objective: To determine the effect of exogenous signal transduction pathway JAK/STAT on apoptosis of human SCC cells induced by 5-ALA-PDT. Methods: Squamous cell carcinoma cell line A431 and COLO-16 cells were cultured in vitro, and then were divided into blank control group, negative control group, PDT group, negative control+PDT group, STAT3 high expression vector group, STAT3-siRNA group, STAT3 high expression vector+PDT group and STAT3-siRNA+ PDT group. The survival rate and apoptosis rate were detected by CCK-8 method and flow cytometry method (FCM). The expression level of mRNA and protein of STAT3 and the downstream gene of Bax and Bcl-2 were detected by qRT-PCR and Western Blot. Results: The apoptosis rate of A431 and COLO-16 cells were 24% and 22% in PDT group, which were higher than in the STAT3 high expression vector+PDT group (11% and 10.5%), but lower than those in the STAT3-siRNA+ PDT group (36% and 39%).The level of STAT3 and Bcl-2 mRNA and protein in the PDT group were higher than those in the STAT3 high expression vector+PDT group, but lower than those in the STAT3-siRNA+ PDT group. The level of BCL-2mRNA and protein in PDT group was lower than that in the STAT3 high expression vector+PDT group, but higher than that in the STAT3-siRNA+ PDT group. Conclusion: Exogenous apoptotic pathway JAK-STAT3 may involved in the apoptosis of squamous cancer cells induced by 5-ALA-PDT. |
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| Keywords: | photodynamic therapy (PDT) 5-aminolevulinic acid skin squamous cell carcinoma JAK/STAT3 |
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