A visualization pipeline for in vivo two-photon volumetric astrocytic calcium imaging |
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| Authors: | Qian Sun Yusi Hu Saiyue Deng Yanyu Xiong Zhili Huang |
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| Affiliation: | 1.Department of Pharmacology, School of Basic Medical Science, Fudan University, Shanghai 200032, China2.State Key Laboratory of Medical Neurobiology, Institutes of Brain Science and Collaborative Innovation Center for Brain Science, Fudan University, Shanghai 200032, China3.Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China |
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| Abstract: | Astrocytes, the multi-functional glial cells with the most abundant population in the brain, integrate information across their territories to regulate neuronal synaptic and cerebrovascular activities. Astrocytic calcium (Ca2+) signaling is the major readout of cellular functional state of astrocytes. The conventional two-photon in vivo imaging usually focuses on a single horizontal focal plane to capture the astrocytic Ca2+ signals, which leaves >80% spatial information undetected. To fully probe the Ca2+ activity across the whole astrocytic territory, we developed a pipeline for imaging and visualizing volumetric astrocytic Ca2+ time-lapse images. With the pipeline, we discovered a new signal distribution pattern from three-dimensional (3D) astrocytic Ca2+ imaging data of mice under isoflurane anesthetic states. The tools developed in this study enable a better understanding of the spatiotemporal patterns of astrocytic activity in 3D space. |
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| Keywords: | astrocyte calcium imaging three-dimensional visualization |
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