| 人脐带间充质干细胞分离培养鉴定及诱导分化实验 |
| |
| 引用本文： | 谢 娜,娄 鸣,饶国洲,朱 勇,唐成芳,王 琳.人脐带间充质干细胞分离培养鉴定及诱导分化实验[J].现代检验医学杂志,2015,0(3):67-70. |
| |
| 作者姓名： | 谢 娜 娄 鸣 饶国洲 朱 勇 唐成芳 王 琳 |
| |
| 作者单位： | 1.西安医学院口腔医学系,西安 710021; 2.西安交通大学口腔医院,西安 710004 |
| |
| 摘 要： | 目的 建立人脐带间充质干细胞(MSCs)分离培养方法,并进行生物学鉴定及定向诱导分化研究。方法 采用酶消化Wharton胶法体外分离培养人脐带干细胞,通过流式细胞仪分析其表面标记分子,并向成骨、成脂方向诱导分化,钙钴法染色检测ALP,茜素红染色检测矿化结节,油红“O”染色检测脂肪滴,进行干细胞的成骨成脂活性鉴定。结果 分离培养的脐带间充质干细胞CD73,CD90和CD105均表达阳性,阳性率为92.45%,95.45%和96.45%,CD34表达阴性,阳性率仅为1.07%。成骨诱导3周后ALP染色胞质内可见黑色颗粒,4周后可见大量矿化结节。成脂诱导2周后细胞内出现大量脂肪小滴,多数细胞形态变圆,油红“O”染色阳性。结论 脐带来源的间充质干细胞具有成骨、成脂多向分化潜能,为临床干细胞治疗研究的种子细胞来源奠定了基础。
|
| 关 键 词： | 脐带 wharton胶 干细胞 诱导分化 |
Isolation,Culture,Identification and Induced Differentiation of Mesenchymal Stem Cells from Human Umbilical Cord |
| |
| XIE Na,LOU Ming,RAO Guo-zhou,ZHU Yong,TANG Cheng-fang,WANG Lin.Isolation,Culture,Identification and Induced Differentiation of Mesenchymal Stem Cells from Human Umbilical Cord[J].Journal of Modern Laboratory Medicine,2015,0(3):67-70. |
| |
| Authors: | XIE Na LOU Ming RAO Guo-zhou ZHU Yong TANG Cheng-fang WANG Lin |
| |
| Affiliation: | 1.Department of Stomatology,Xi'an Medical College,Xi'an 710021,China;
2.Hospital of Stomatology Xi'an Jiaotong University,Xi'an 710004,China |
| |
| Abstract: | Objective To establish the method of isolation,culture and identify biological characterization of mesenchymal stem cells from human umbilical cord(hUCMSCs); and study their multiple differentiation potency.Methods Stem cells from human umbilical cord were cultured by enzyme Wharton jelly method in vitro.The surface markers were identified by flow cytometry.Multi-differentiation capacity was identified by osteogenic and adipogenic differentiation.ALP was detected with Calcium cobalt staining.The mineralized ability in vitro was measured with Alizarin red staining.Theadipocyte differentiation ability was measured with oil red-O staining.Results Flow cytometry analysis revealed that CD73(92.45%),CD90(95.45%)and CD105(96.45%)were highly expressed on these cells'surface,while CD34(1.07%)were negative expressed.Cells were cultured with induced-osteogenic medium after 3 weeks,ALP staining in the cytoplasm of black particles,and a large amount of mineralized nodules within cells was observed after 4 weeks.Cells were cultured with induced-adipogenic medium after 2 weeks,the majority of these cells were round,oil red O staining of lipid droplets generated within cells was observed.Conclusion Mesenchymal stem cells from human umbilical cord have the potential of multi-directional differentiation.These cells could be induced to differentiate into adipocytes and osteoblasts,which laid the foundation for clinical stem cell therapy research source ofseed cells. |
| |
| Keywords: | |
| 本文献已被 万方数据 等数据库收录！ |
| 点击此处可从《现代检验医学杂志》浏览原始摘要信息 |
|
点击此处可从《现代检验医学杂志》下载全文 |
|
