| p53基因codon 72多态性与乳腺癌的相关性研究* |
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| 引用本文： | 周晓颖,张丽娜,赵妍蕊,郑红,陈可欣.p53基因codon 72多态性与乳腺癌的相关性研究*[J].中国肿瘤临床,2010,37(9):499-503. |
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| 作者姓名： | 周晓颖 张丽娜 赵妍蕊 郑红 陈可欣 |
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| 作者单位： | 作者单位:乳腺癌防治教育部重点实验室,天津市肿瘤防治重点实验室,天津医科大学附属肿瘤医院肿瘤流行病与生物统计学研究室(天津市 300060) |
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| 基金项目： | 国家自然科学基金资助 |
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| 摘 要： | 目的:研究p53基因codon 72 多态性与乳腺癌患者的年龄、病理分期、淋巴结转移、雌激素受体（ER）、孕激素受体（PR）、c-erbB-2、P53蛋白表达情况的相关性。方法:TaqMan探针方法检测277 例乳腺癌患者血液标本的p53基因codon 72多态性。免疫组化SP法检测匹配肿瘤组织中ER、PR、c-erbB-2 和P53蛋白的表达情况。SPSS16.0 软件行统计学分析，p53基因多态性与病理学特征关系用χ2检验，非条件Logistic回归分析基因多态性与ER、PR、c-erbB-2、P53蛋白表达的相关性，计算OR值及其95% 可信区间（95% CI）。 P<0.05为差异有统计学意义。结果:p53基因codon 72基因型为CC/CG/GG，频率分别为22.0% 、51.3% 和26.7% ，携带CC、CG、GG基因型的患者发病年龄逐渐降低，但无统计学差异；p53基因codon 72多态性与临床病理学特征无关，与ER、PR、c-erbB-2 和P53蛋白表达无相关性（P>0.05）。 肿瘤组织P53蛋白表达与ER、PR、c-erbB-2 蛋白表达密切相关（χ2=13.492，P=0.000；χ2=3.970，P=0.046；χ2=17.956，P=0.000）。 结论:p53基因codon 72多态性与P53蛋白表达及病理学特征无相关性，P53蛋白表达与ER、PR、c-erbB-2 蛋白表达关系密切。p53基因codon 72基因型与患者发病年龄的关系有待扩大样本量进一步研究。
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| 关 键 词： | p53codon 72 乳腺癌 免疫组化 |
| 收稿时间： | 2010-01-10 |
Study on p53 Codon 72 Polymorphism in 277 Patients with Breast Cancer |
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| Affiliation: | Department of Epidemiology and Biostatistics, Cancer Institute and Hospital of Tianjin Medical University, Tianjin 300060, China |
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| Abstract: | Objective: To investigate the correlation of p53codon 72polymorphism with clinicopathologic features of breast cancer patients including age, tumor staging, lymph node status, receptor status of estrogen and progestin, expres-sion of P 53protein and c-erbB- 2. Methods:Blood samples were collected from 277 women with primary breast cancer. Taq-Man was used to determine the genotypes of p53codon 72polymorphism. Expression of ER, PR, c-erbB- 2 and P 53pro-teins were detected with immunohistochemistry. SPSS 16.0 software was employed for statistical analysis and the relation -ship of p 53polymorphism with all of the clinicopathologic parameters was evaluated with χ2 test. Furthermore, the associa -tion of p 53polymorphism with ER, PR, c-erbB- 2 and P 53protein expression was analyzed by logistic regression analysis. Results: The frequency of CC, CG, and GG at p 53codon 72was 22.0%,51.3%, and26.7%, respectively. No correlation was found between genotype distribution and clinicopathologic parameters of disease outcome. There was no correlation between p 53condon 72polymorphisms and the expression of ER, PR, c-erbB-2, or P 53proteins (P>0.05). P 53protein ex-pression was associated with expression of ER, PR, and c-erbB-2 proteins (χ2=13.492 , P=0.000 ; χ2=3.970 , P=0.046 ; χ2=17.956 , P=0.000 ). Conclusion:There is no correlation between p53codon 72polymorphism and clinicopathologic parame -ters of breast cancer. P 53protein is significantly associated with ER, PR and c-erbB-2 expression. Further study using a larger sample is warranted. |
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| Keywords: | p53 codon 72 |
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