| 产金属β-内酰胺酶铜绿假单胞菌的耐药性和基因检测 |
| |
| 引用本文： | 谭萍,祝玲玲,张松,朱平安,邹汉良,赖秀花. 产金属β-内酰胺酶铜绿假单胞菌的耐药性和基因检测[J]. 中国感染控制杂志, 2008, 7(2): 114-116 |
| |
| 作者姓名： | 谭萍 祝玲玲 张松 朱平安 邹汉良 赖秀花 |
| |
| 作者单位： | 深圳市第七人民医院,广东,深圳,518081 |
| |
| 摘 要： | 目的研究某地区临床分离的铜绿假单胞菌产金属β-内酰胺酶株主要基因型及其耐药性。方法以聚合酶链反应（PCR）法检测该市3所医院2002年8月—2007年6月分离的100株耐亚胺培南和（或）头孢他啶铜绿假单胞菌中产金属β-内酰胺酶基因,并用二倍琼脂稀释法检测10种抗菌药物对产金属酶株的体外最低抑菌浓度（MIC）。结果共检出9株金属酶阳性菌株,其中3株IMP-1扩增阳性;6株VIM扩增阳性,经基因测序确认为VIM-2型。产酶株对头孢哌酮、头孢吡肟、亚胺培南、复方磺胺甲口恶唑全部耐药,部分对阿米卡星、环丙沙星较为敏感。结论该地区铜绿假单胞菌流行株金属酶基因型主要为VIM-2型和IMP-1型;其耐药性强,临床应根据药敏结果选择药物联合使用,以达到最佳抗菌效果。
|
| 关 键 词： | 金属β-内酰胺酶 铜绿假单胞菌 基因型 抗药性 微生物 聚合酶链反应 |
| 文章编号： | 1671-9638(2008)02-0114-04 |
| 修稿时间： | 2007-12-17 |
Analysis of genotypes and antimicrobial resistance of metallo-beta-lacta-mase-producing Pseudomonas aeruginosa |
| |
| TAN Ping,ZHU Ling-ling,ZHANG Song,ZHU Ping-an,ZHOU Han-liang,LAI Xiu-hua. Analysis of genotypes and antimicrobial resistance of metallo-beta-lacta-mase-producing Pseudomonas aeruginosa[J]. Chinese Journal of Infection Control, 2008, 7(2): 114-116 |
| |
| Authors: | TAN Ping ZHU Ling-ling ZHANG Song ZHU Ping-an ZHOU Han-liang LAI Xiu-hua |
| |
| Affiliation: | (The Seventh People＇s Hospital of Shengzhen ,Shengzhen 518081, China) |
| |
| Abstract: | Objective To study the genotypes and antimicrobial resistance of metallo-beta-lactamase-producing Pseudomonas aeruginosa isolated from clinical hospitals in an area. Methods The genes of metallo-beta-lactamase were detected by polymerase chain reaction （PCR） in 100 imipenem and/or ceftazidime-resistant strains of Pseudomonas aeruginosa isolated from 3 hospitals from August, 2002 to June, 2007, and the minimum inhibitory concentration of 10 kinds of antimicrobial agents were determined by agar dilution method in the metallo-beta-lactamase- producing strains. Results Nine strains produced metallo-beta-lactamases, 3 of which were positive in PCR analysis with blaIMP-1 primers, and 6 were blaVIM and identified as VIM-2 by DNA sequence. All the metallo-beta-lacta- mase- producing strains were resistant to cefoperazone, cefepime, imipenem, sulfamethoxazole-trimethoprim, partly resistant to amikacin and ciprofloxacin. Conclusion The main genotypes of metallo-beta-lactamase in epidemic strains of Pseudomonas aeruginosa were VIM-2 and IMP-1, these strains had strong drug-resistance, it is important to choose antimicrobial agents according to antimicrobial susceptibility test. |
| |
| Keywords: | metallo-beta-lactamase Pseudomonasaeruginosa genotype drug-resistance, microbial polymerasechain reaction |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
