| miR-195过表达促进人神经胶质瘤细胞T98G凋亡 |
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| 引用本文： | 赵志强,阴彬,彭小忠. miR-195过表达促进人神经胶质瘤细胞T98G凋亡[J]. 基础医学与临床, 2012, 32(7): 783-787 |
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| 作者姓名： | 赵志强 阴彬 彭小忠 |
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| 作者单位： | 中国医学科学院基础医学研究所北京协和医学院基础学院医学分子生物学国家重点实验室,北京,100005 |
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| 摘 要： | 目的检测过表达miR-195对人神经胶质瘤细胞T98G增殖的影响,探讨miRNA-195在胶质瘤发病过程中可能机制。方法用合成的miR-195模拟物,转染miR-195表达水平较低的人神经胶质瘤细胞T98G,用MTT、流式细胞术和TUNEL法分别检测T98G细胞增殖、细胞周期和凋亡,用Western blot检测细胞BCL-2的表达。结果 miR-195模拟物转染T98G后,成熟miR-195明显升高(P<0.05)。在T98G中过表达miR-195后,可明显抑制细胞增殖,过表达miR-195能促进T98G细胞的凋亡和下调细胞BCL-2的表达。结论过表达miR-195能显著促进T98G细胞凋亡,提示miR-195可能对胶质瘤的治疗起作用。
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| 关 键 词： | miR-195 T98G细胞 细胞增殖 凋亡 BCL-2 |
Overexpression of miR-195 promotes apoptosis in human glioma T98G cells |
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| ZHAO Zhi-qiang , YIN Bin , PENG Xiao-zhong. Overexpression of miR-195 promotes apoptosis in human glioma T98G cells[J]. Basic Medical Sciences and Clinics, 2012, 32(7): 783-787 |
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| Authors: | ZHAO Zhi-qiang YIN Bin PENG Xiao-zhong |
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| Affiliation: | (the National Laboratory of Medical Molecular Biology,Institute of Basic Medical Sciences,CAMS,School of Basic Medicine,PUMC,Beijing 100005,China) |
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| Abstract: | Objective To investigate the influence of overexpression of miR-195 on cell proliferation in glioma T98G cells and explore the function of miR-195 in glioma.Methods T98G cells with lower expression of miR-195 were transfected with synthesized miR-195 mimics,then MTT assay,flow cytometry and TUNEL assay were performed to analyse the alterations of cellular phenotype,Western blot was performed to examine the expression level of BCL-2.Results Mature miR-195 increased significantly after miR-195 mimics transfection(P<0.05).Overexpression of miR-195 induced significant cell proliferation suppression,and up-regulated miR-195 promoted apoptosis and reduced the expression of BCL-2 protein in T98G cells.Conclusions Overexpression of miR-195 significantly promotes apoptosis of T98G cells,suggesting that miR-195 may serve as a potential therapeutic agent for glioma. |
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| Keywords: | miR-195 T98G cells cell proliferation apoptosis BCL-2 |
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