| 转录因子7类似物2在胃癌中的表达及其与铂类药物耐药性的关系 |
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| 引用本文： | 付翠群,丁西平,沈国栋,侯冠峰,戴朦,沈干,胡世莲.转录因子7类似物2在胃癌中的表达及其与铂类药物耐药性的关系[J].中国临床保健杂志,2018(1):92-95. |
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| 作者姓名： | 付翠群 丁西平 沈国栋 侯冠峰 戴朦 沈干 胡世莲 |
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| 作者单位： | 中国科学技术大学附属第一医院(安徽省立医院)安徽省老年医学研究所肿瘤免疫与营养治疗安徽省重点实验室安徽省循证医学中心; |
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| 基金项目： | 国家自然科学基金(81071808)；安徽省科技攻关项目(1501041142)；肿瘤免疫与营养治疗安徽省重点实验室绩效项目(1606c08236) |
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| 摘 要： | 目的研究转录因子7类似物2(TCF7L2)基因在胃癌中的表达情况及其与胃癌化疗耐药的关系。方法采用qRT-PCR方法检测15例胃癌及其癌旁组织中TCF7L2的mRNA量,western blot实验检测胃癌细胞株SGC-7901、HSC44、44AS3、N87、MKN45、MGC803与永生化正常胃黏膜上皮细胞GES-1中TCF7L2蛋白表达量。通过基因稳定转染技术构建高表达TCF7L2的SGC-7901/TCF7L2细胞株和SGC-7901/CON(对照组)细胞株,用细胞计数法分别检测两者对顺铂和卡铂的耐药情况。结果 TCF7L2在胃癌组织中的表达显著高于癌旁正常组织,在胃癌细胞株中的表达水平也明显高于对照细胞GES-1。高表达TCF7L2的SGC-7901/TCF7L2细胞株对顺铂与卡铂的耐受性明显强于对照细胞株。结论 TCF7L2基因表达与胃癌的发生发展有正相关关系;TCF7L2高表达可能是胃癌对铂类药物化疗耐受的原因之一。
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| 关 键 词： | 胃肿瘤 TCF7L2 药物耐受性 卡铂 顺铂 |
| 收稿时间： | 2016/12/1 0:00:00 |
Expression of TCF7L2 and its relationship with platinum drug resistance in gastric cancer |
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| Fu Cuiqun,Ding Xiping,Shen Guodong,Hou Guanfeng,Dai Meng,Shen Gan and Hu Shilian.Expression of TCF7L2 and its relationship with platinum drug resistance in gastric cancer[J].Chinese JOurnal of Clinical Healthcare,2018(1):92-95. |
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| Authors: | Fu Cuiqun Ding Xiping Shen Guodong Hou Guanfeng Dai Meng Shen Gan and Hu Shilian |
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| Affiliation: | The First Affiliated Hospital of University of Science and Technology of China,Anhui Institute of Gerontology,Anhui Provincial Key Laboratory of Tumor Immunotherapy and Nutrition Therapy,Anhui Evidence-based Medicine Center,Hefei 230001,China,The First Affiliated Hospital of University of Science and Technology of China,Anhui Institute of Gerontology,Anhui Provincial Key Laboratory of Tumor Immunotherapy and Nutrition Therapy,Anhui Evidence-based Medicine Center,Hefei 230001,China,The First Affiliated Hospital of University of Science and Technology of China,Anhui Institute of Gerontology,Anhui Provincial Key Laboratory of Tumor Immunotherapy and Nutrition Therapy,Anhui Evidence-based Medicine Center,Hefei 230001,China,The First Affiliated Hospital of University of Science and Technology of China,Anhui Institute of Gerontology,Anhui Provincial Key Laboratory of Tumor Immunotherapy and Nutrition Therapy,Anhui Evidence-based Medicine Center,Hefei 230001,China,The First Affiliated Hospital of University of Science and Technology of China,Anhui Institute of Gerontology,Anhui Provincial Key Laboratory of Tumor Immunotherapy and Nutrition Therapy,Anhui Evidence-based Medicine Center,Hefei 230001,China,The First Affiliated Hospital of University of Science and Technology of China,Anhui Institute of Gerontology,Anhui Provincial Key Laboratory of Tumor Immunotherapy and Nutrition Therapy,Anhui Evidence-based Medicine Center,Hefei 230001,China and The First Affiliated Hospital of University of Science and Technology of China,Anhui Institute of Gerontology,Anhui Provincial Key Laboratory of Tumor Immunotherapy and Nutrition Therapy,Anhui Evidence-based Medicine Center,Hefei 230001,China |
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| Abstract: | Objective To study the expression of TCF7L2 gene in gastric cancer and its relationship with platinum resistance.Methods TCF7L2 mRNA of 15 gastric cancer tissues and adjacent cancer tissues were detected by qRT-PCR,and TCF7L2 protein expression of gastric cancer cell lines SGC-7901,HSC44,4AS3,N87,MKN45,MGC803,and immortalized normal gastric epithelial cells GES-1 were detected by western blot.The high TCF7L2 protein expression of gastric cancer cell line SGC-7901/TCF7L2 and idling control SGC-7901 cell line (SGC-7901/CON) by stable plasmid transfection technique,the resistance of cisplatin and carboplatin detected by cell counting.Results The expression level of TCF7L2 was significantly higher in the tumors than that in the non-carcinomatous gastric tissues.The expression levels of TCF7L2 in gastric cancer cell lines were also significantly higher than control cell line GES-1.The tolerance of resistant of SGC-7901/TCF7L2 cell line with high expression of TCF7L2 to cisplatin and carboplatin were better than than the control cell lines.Conclusion There is a positive relationship between expression of TCF7L2 and occurrence and development of gastric carcinoma.It may be one of the reasons for its tolerance to platinum drugs. |
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