| 区域淋巴结切除对小鼠结直肠肿瘤CEA、VEGF-C表达的影响 |
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| 引用本文： | 万首伟,' target='_blank'>,黄桂林,韩丹青,侯吉学.区域淋巴结切除对小鼠结直肠肿瘤CEA、VEGF-C表达的影响[J].现代肿瘤医学,2023,0(11):1992-1995. |
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| 作者姓名： | 万首伟 ' target='_blank'> 黄桂林 韩丹青 侯吉学 |
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| 作者单位： | 1.石河子大学医学院，新疆 石河子 832000;2.石河子市人民医院普外科，新疆 石河子 832000;3.石河子大学医学院第一附属医院普外二科，新疆 石河子 832000 |
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| 基金项目： | 兵团科技计划项目（编号:2022ZD025） |
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| 摘 要： | 目的:探讨区域淋巴结切除对小鼠结直肠肿瘤CEA、VEGF-C表达的影响。方法:将60只健康BALB/c小鼠分为3组，虚拟切口+PBS注射15只（A组），虚拟切口+CT26.WT注射30只（B组），模型组（区域淋巴结切除）+CT26.WT注射15只（C组）。再将B组分为B1组15只（未行区域淋巴结切除）、B2组15只（肿瘤埋植7天后行区域淋巴结切除）。用ELISA法检测各组小鼠血清CEA、VEGF-C浓度，并对各组小鼠的瘤块称重及测算体积。结果:A组、B1组、B2组、C组小鼠血清CEA浓度分别为（602.6±52.2）pg/mL、（640.3±37.6）pg/mL、（676.5±56.6）pg/mL、（698.9±45.7）pg/mL，四组小鼠血清VEGF-C浓度分别为（153.5±13.0）pg/mL、（164.6±14.6）pg/mL、（177.1±15.4）pg/mL、（186.0±17.2）pg/mL。B组、C组的小鼠血清CEA、VEGF-C浓度相比A组均有不同程度的升高（P<0.05）；与B1组相比，B2组、C组小鼠血清CEA、VEGF-C浓度均升高（P<0.05）；瘤块重量及体积均明显增大（P<0.05）。结论:区域淋巴结切除可能会使小鼠肿瘤CEA及VEGF-C的表达上调，促进移植瘤的生长和侵袭。
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| 关 键 词： | 区域淋巴结切除 CT26.WT CEA VEGF-C |
Effects of regional lymphadenectomy on expression of CEA and VEGF-C in mice colorectal tumors |
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| WAN Shouwei,' target='_blank'>,HUANG Guilin,HAN Danqing,HOU Jixue.Effects of regional lymphadenectomy on expression of CEA and VEGF-C in mice colorectal tumors[J].Journal of Modern Oncology,2023,0(11):1992-1995. |
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| Authors: | WAN Shouwei ' target='_blank'> HUANG Guilin HAN Danqing HOU Jixue |
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| Affiliation: | 1.Medical College of Shihezi University,Xinjiang Shihezi 832000,China;2.Department of General Surgery,Shihezi People's Hospital,Xinjiang Shihezi 832000,China;3.The Second Department of General Surgery,First Affiliated Hospital of Medical College of Shihezi University,Xinjiang Shihezi 832000,China. |
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| Abstract: | Objective:To investigate the effects of regional lymphadenectomy on expression of CEA and VEGF-C in mice colorectal tumors.Methods:60 healthy BALB/c mice were divided into three groups:Virtual incision+PBS injection，15 mice(group A)，virtual incision+CT26.WT injection，30 mice(group B),and model group+CT26.WT injection，15 mice (group C).The model group mice were excised regional lymph nodes.The mice in group B were further divided into two groups:Group B1 (No regional lymphadenectomy was performed) and group B2(Regional lymphadenectomy was performed after 7 days of tumor implantation) with 15 mice in each group.The concentrations of CEA and VEGF-C in each group were detected by ELISA,and the tumor masses were weighed and measured.Results:The concentrations of CEA in group A,B1,B2 and C were (602.6±52.2) pg/mL,(640.3±37.6) pg/mL,(676.5±56.6) pg/mL and (698.9±45.7) pg/mL,VEGF-C concentrations in group A,B1,B2 and C were (153.5±13.0) pg/mL,(164.6±14.6) pg/mL,(177.1±15.4) pg/mL,(186.0±17.2) pg/mL.The concentrations of CEA and VEGF-C in group B and C were higher than those in group A（P<0.05）.Compared with group B1,the concentrations of CEA and VEGF-C in group B2 and group C were increased(P<0.05).The weight and volume of tumor mass were larger than those in group B1(P<0.05).Conclusion:Regional lymphadenectomy can up-regulate the expression of CEA and VEGF-C in mouse tumors,promote the growth and invasion of transplanted tumor. |
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| Keywords: | regional lymphadenectomy CT26 WT CEA VEGF-C |
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