| MTHFD1L对舌鳞癌细胞增殖、凋亡和体内成瘤的影响及机制研究 |
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| 引用本文： | 徐志铭,白玉婷,周晓凯,陈雨昕,孟 箭,' target='_blank'>.MTHFD1L对舌鳞癌细胞增殖、凋亡和体内成瘤的影响及机制研究[J].现代肿瘤医学,2023,0(2):203-210. |
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| 作者姓名： | 徐志铭 白玉婷 周晓凯 陈雨昕 孟 箭 ' target='_blank'> |
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| 作者单位： | 1.徐州医科大学徐州临床学院，江苏 徐州 221000;2.武汉大学口腔医学院，湖北 武汉 430000;3.徐州医科大学口腔医学院，江苏 徐州 221000;4.徐州市中心医院，江苏 徐州 221000 |
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| 基金项目： | 江苏省卫生计生委科研资助项目（编号:H2017080）；江苏省徐州市科技计划项目（编号:KC21187） |
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| 摘 要： | 目的:探讨亚甲基四氢叶酸脱氢酶1（MTHFD1L）敲减对Cal27细胞增殖、克隆形成、凋亡、成瘤的影响及机制。方法:从TCGA和GTEx数据库下载相关数据，比较头颈鳞癌（HNSC）和正常组织中MTHFD1L表达差异和生存分析，并应用GEPIA数据库验证差异表达及预测生存曲线。利用慢病毒小干扰RNA技术敲减MTHFD1L表达，通过实时荧光定量聚合酶链反应（RT-qPCR）和蛋白质印迹分析（Western blot）检测MTHFD1L敲减效率。Celigo细胞计数法、MTT法、平板克隆实验、Annexin V-APC单染法分别研究敲减MTHFD1L对Cal27细胞增殖、克隆形成和凋亡的影响。经腋窝下注射Cal27细胞建立裸鼠舌癌模型，比较瘤体重量和体积，并用活体成像仪比较荧光强度。RT-qPCR和Western blot检测p38α、IL1α表达水平。结果:MTHFD1L在HNSC中的表达量高于正常组织，且MTHFD1L高表达与预后不良有关。MTHFD1L敲减率达95.1%，Celigo细胞计数法、MTT法、平板克隆结果显示MTHFD1L敲减后Cal27细胞增殖速率和克隆形成能力受到明显抑制。Annexin V-APC单染法结果提示MTHFD1L敲除明显促进Cal27细胞凋亡。接种经MTHFD1L敲减Cal27细胞的裸鼠瘤体体积、重量、荧光强度及成瘤率明显降低。Cal27细胞经MTHFD1L敲减后，p38α蛋白和mRNA表达量明显降低，而IL1α明显升高。结论:MTHFD1L基因敲减明显抑制舌鳞癌细胞增殖，诱导凋亡，并抑制小鼠体内成瘤。
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| 关 键 词： | MTHFD1L 舌鳞状细胞癌 增殖 凋亡 p38α IL1α |
Effects of MTHFD1L on proliferation,apoptosis and tumorigenesis of tongue squamous cell carcinoma cells and its mechanism |
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| XU Zhiming,BAI Yuting,ZHOU Xiaokai,CHEN Yuxin,MENG Jian,' target='_blank'>.Effects of MTHFD1L on proliferation,apoptosis and tumorigenesis of tongue squamous cell carcinoma cells and its mechanism[J].Journal of Modern Oncology,2023,0(2):203-210. |
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| Authors: | XU Zhiming BAI Yuting ZHOU Xiaokai CHEN Yuxin MENG Jian ' target='_blank'> |
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| Affiliation: | 1.Xuzhou Clinical College of Xuzhou Medical University,Jiangsu Xuzhou 221000,China;2.School of Stomatology,Wuhan University,Hubei Wuhan 430000,China;3.School of Stomatology,Xuzhou Medical University,Jiangsu Xuzhou 221000,China;4.Xuzhou Central Hospital,Jiangsu Xuzhou 221000,China. |
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| Abstract: | Objective:To investigate the effect of methylenetetrahydrofolate dehydrogenase 1 (MTHFD1L) knockdown on the proliferation,clone formation,apoptosis and tumorigenesis of Cal27 and its mechanism.Methods:Data related to head and neck squamous cell carcinoma (HNSC) was downloaded from TCGA and GTEx databases to compare the difference of MTHFD1L expression and survival analysis between HNSC and normal tissues.GEPIA database was used to verify differential expression and predict survival curves.The expression of MTHFD1L was knocked down by lentivirus small interfering RNA technology,and the knockdown efficiency of MTHFD1L was detected by RT-qPCR and Western blot analysis.The effects of MTHFD1L knockdown on Cal27 cell proliferation,clonal formation and apoptosis were investigated by Celigo cell count assay,MTT assay,plate cloning assay and Annexin V-APC staining,respectively.The nude mouse tongue cancer model was established by injecting Cal27 cells into the armpit.The weight,volume and fluorescence intensity were compared.The expression levels of p38α and IL1α were detected by RT-qPCR and Western blot.Results:The expression of MTHFD1L in HNSC was higher than that in normal tissues,and the high expression of MTHFD1L was associated with poor prognosis.MTHFD1L knockdown rate was 95.1%.The results of Celigo cell counting,MTT and plate cloning showed that MTHFD1L knockdown inhibited the proliferation rate and clonal formation ability of Cal27.Annexin V-APC staining showed that MTHFD1L knockdown significantly promoted apoptosis of Cal27.The tumor volume,weight,fluorescence intensity and tumor formation rate of nude mice inoculated with MTHFD1L knockdown Cal27 cells decreased significantly.After MTHFD1L knockdown,the expression of p38α and mRNA in Cal27 cells decreased significantly,while the expression of IL1α increased significantly.Conclusion:MTHFD1L knockdown inhibited tongue squamous cell carcinoma cell proliferation,induced apoptosis,and inhibited tumor formation in mice. |
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| Keywords: | MTHFD1L tongue squamous cell carcinoma proliferation apoptosis p38α IL1α |
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