The endomembrane requirement for cell surface repair |
| |
| Authors: | McNeil Paul L Miyake Katsuya Vogel Steven S |
| |
| Affiliation: | Department of Cellular Biology and Anatomy, Medical College of Georgia, Augusta, GA 30912, USA. pmcneil@mail.mcg.edu |
| |
| Abstract: | ![]()
The capacity to reseal a plasma membrane disruption rapidly is required for cell survival in many physiological environments. Intracellular membrane (endomembrane) is thought to play a central role in the rapid resealing response. We here directly compare the resealing response of a cell that lacks endomembrane, the red blood cell, with that of several nucleated cells possessing an abundant endomembrane compartment. RBC membrane disruptions inflicted by a mode-locked Ti:sapphire laser, even those initially smaller than hemoglobin, failed to reseal rapidly. By contrast, much larger laser-induced disruptions made in sea urchin eggs, fibroblasts, and neurons exhibited rapid, Ca(2+)-dependent resealing. We conclude that rapid resealing is not mediated by simple physiochemical mechanisms; endomembrane is required. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
