Protective effects of thymoquinone on experimental testicular ischaemia–reperfusion injury: an apoptotic,proliferative and biochemical study |
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| Authors: | M. Erboga C. Aktas O. Kurt R. Uygur V. Caglar B. C. Turan B. Topcu Z. Fidanol Erboga A. Gurel O. A. Ozen |
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| Affiliation: | 1. Department of Histology and Embryology, Faculty of Medicine, University of Namik Kemal, Tekirdag, Turkey;2. Department of Urology, Faculty of Medicine, University of Namik Kemal, Tekirdag, Turkey;3. Department of Anatomy, Faculty of Medicine, University of Namik Kemal, Tekirdag, Turkey;4. Department of Anesthesiology and Reanimation, Faculty of Medicine, University of Namik Kemal, Tekirdag, Turkey;5. Department of Biostatistics, Faculty of Medicine, University of Namik Kemal, Tekirdag, Turkey;6. Department of Biochemistry, Faculty of Medicine, University of Namik Kemal, Tekirdag, Turkey |
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| Abstract: | The objective of this study was to examine the effects of thymoquinone (TQ), which has antioxidant properties in the experimental testicular I/R model in rats in terms of its anti‐apoptotic, proliferative and biochemical attributes. In our study, 24 male rats were divided into three groups: control group, I/R group and I/R+TQ group. Testicular torsion was created by rotating the left testis 720° in a clockwise direction. The ischaemia period was 4 h, and an orchiectomy was performed after 4 h of detorsion. Spermatogenesis and the mean seminiferous tubule diameter were significantly decreased in the I/R groups compared to the control group. Furthermore, TQ‐treated animals displayed an improved histological appearance in the I/R group. It was also observed that treatment with TQ increased the activity of PCNA, which decreased as a result of I/R, and this treatment also reduced the number of TUNEL‐positive cells. The I/R+TQ group showed a decrease in malondialdehyde levels and an increase in the activities of superoxide dismutase, catalase and glutathione peroxidase in comparison with the I/R group. It could be concluded that cytoprotective effects of TQ on the I/R testicles are via reduction of apoptosis, oxidative stress and lipid peroxidation. |
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| Keywords: | Apoptosis ischaemia and reperfusion oxidative stress testis thymoquinone |
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