| 重组人红细胞生成素对癫痫持续状态大鼠海马p-Akt和caspase-9的影响及作用机制 |
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| 引用本文： | 王维平,史志勤,于江华,郭力,王乐,韩东亮,袁栋才,臧颖卓. 重组人红细胞生成素对癫痫持续状态大鼠海马p-Akt和caspase-9的影响及作用机制[J]. 南方医科大学学报, 2010, 30(1): 64 |
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| 作者姓名： | 王维平 史志勤 于江华 郭力 王乐 韩东亮 袁栋才 臧颖卓 |
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| 作者单位： | 河北医科大学第二医院神经内科,河北石家庄,050000;北京天坛医院神经介入科,北京,100050;衡水市哈励逊国际和平医院,河北衡水,053000 |
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| 基金项目： | 河北省自然科学基金(C2007000852);;河北省科技攻关项目(07276101D-21) |
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| 摘 要： | 目的观察重组人红细胞生成素(rHuEPO)对戊四氮(PTZ)点燃的癫痫持续状态(status epilepticus,SE)的SD大鼠海马神经元磷酸化蛋白激酶B(p-PKB/p-Akt)和半胱氨酸天冬氨酸蛋白酶9(Caspase-9)表达的影响,应用磷脂酰肌醇3激酶(PI3K)抑制剂LY294002进一步探讨rHuEPO作用的可能机制。方法采用PTZ点燃大鼠SE模型,将175只大鼠随机分为正常对照组(给予生理盐水腹腔注射)、PTZ组(腹腔注射PTZ点燃SE发作后30min腹腔注射生理盐水)、rHuEPO组(SE发作后30min腹腔注射rHuEPO5000U/kg)、LY294002组(SE发作后10min脑室注射5μlLY294002,SE发作后30min腹腔注射rHuEPO5000U/kg)、二甲基亚砜(DMSO)组(SE发作后10min脑室注射5μlDMSO,SE发作后30min腹腔注射rHuEPO5000U/kg),并给予相应处理。检测大鼠行为学和脑电图的改变,免疫组织化学法观察p-Akt、caspase-9的表达,RT-PCR方法检测各组大鼠海马caspase-9mRNA的表达;Westernblot...
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| 关 键 词： | 癫痫持续状态 重组人红细胞生成索 磷脂酰肌醇3激酶/蛋白激酶B 半胱氨酸天冬氨酸蛋白酶9 |
Effect of recombinant human erythropoietin on hippocampal p-Akt and caspase-9 expressions in rats with status epilepticus and the mechanism |
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| WANG Wei-ping,SHI Zhi-qin,YU Jiang-hua,GUO Li,WANG Le,HAN Dong-liang,YUAN Dong-cai,ZANG Ying-zhuo. Effect of recombinant human erythropoietin on hippocampal p-Akt and caspase-9 expressions in rats with status epilepticus and the mechanism[J]. Journal of Southern Medical University, 2010, 30(1): 64 |
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| Authors: | WANG Wei-ping SHI Zhi-qin YU Jiang-hua GUO Li WANG Le HAN Dong-liang YUAN Dong-cai ZANG Ying-zhuo |
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| Affiliation: | WANG Wei-ping1,SHI Zhi-qin1,YU Jiang-hua2,GUO Li1,WANG Le3,HAN Dong-liang3,YUAN Dong-cai3,ZANG Ying-zhuo1 1Department of Neurology,Second Hospital of Hebei Medical University,Shijiazhuang 050000,China,2Department of Neuroradiology,Beijing Tiantan Hospital,Beijing 100050,3Harrison International Peace Hospital,Hengshui 053000 |
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| Abstract: | Objective To observe the effect of recombinant human erythropoietin (rhuEPO) on p-Akt and caspase-9 expressions in the hippocampus of rats with status epilepticus (SE) and explore the neuroprotective mechanism of rhuEPO. Methods Adult male SD rats were randomized into control, PTZ, rHuEPO, LY294002 group, and DMSO groups and treated with normal saline (NS), PTZ, PTZ+rHuEPO, PTZ+LY294002+rHuEPO, and PTZ+DMSO+rHuEPO, respectively. The behavioral and electroencephalogram (EEG) changes of the rats were recorded... |
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| Keywords: | status epilepticus recombinant human erythropoietins PI3K/Akt caspase-9 |
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