| 薯蓣皂苷对高糖诱导的视网膜色素上皮细胞损伤的保护作用 |
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| 引用本文： | 张新霞,王小敏,陆丽红,王保君,狄文玉. 薯蓣皂苷对高糖诱导的视网膜色素上皮细胞损伤的保护作用[J]. 眼科新进展, 2022, 0(4): 278-283. DOI: 10.13389/j.cnki.rao.2022.0056 |
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| 作者姓名： | 张新霞 王小敏 陆丽红 王保君 狄文玉 |
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| 作者单位： | 453100 河南省新乡市，新乡医学院第一附属医院眼科（张新霞，王小敏，陆丽红，王保君）；453100 河南省新乡市，新乡医学院第一附属医院病理科（狄文玉） |
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| 摘 要： | 目的 探讨薯蓣皂苷(Dio)对高糖(HG)诱导的视网膜色素上皮(RPE)细胞损伤的保护作用,并分析其机制.方法 用细胞计数试剂8(CCK-8)法筛选葡萄糖浓度和无细胞毒性的Dio剂量范围.将ARPE-19细胞分为对照组(5 mmol·L-1葡萄糖处理48 h)、模型组(50 mmol·L-1葡萄糖处理48 h)和低、中...
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| 关 键 词： | 薯蓣皂苷 糖尿病视网膜病变 视网膜色素上皮细胞 高糖 氧化应激 细胞凋亡 |
Protective effect of diosgenin on retinal pigment epithelial cell injury induced by high glucose |
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| ZHANG Xinxia1,WANG Xiaomin1,LU Lihong1,WANG Baojun1,DI Wenyu2. Protective effect of diosgenin on retinal pigment epithelial cell injury induced by high glucose[J]. Recent Advances in Ophthalmology, 2022, 0(4): 278-283. DOI: 10.13389/j.cnki.rao.2022.0056 |
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| Authors: | ZHANG Xinxia1 WANG Xiaomin1 LU Lihong1 WANG Baojun1 DI Wenyu2 |
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| Affiliation: | 1.Department of Ophthalmology,the First Affiliated Hospital of Xinxiang Medical University,Xinxiang 453100,Henan Province,China2.Department of Pathology,the First Affiliated Hospital of Xinxiang Medical University,Xinxiang 453100,Henan Province,China |
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| Abstract: | Objective To investigate the protective effect of diosgenin (Dio) on retinal pigment epithelial (RPE) cell injury induced by high glucose (HG), and to analyze its mechanism. Methods Glucose concentration and non-cytotoxic Dio dose range were screened by CCK-8 assay. ARPE-19 cells were divided into the control group (treated with 5 mmol·L-1 glucose for 48 h), model group (treated with 50 mmol·L-1 glucose for 48 h), and low-, medium- and high-dose Dio groups (treated with 50 mmol·L-1 glucose combined with 0.5 mmol·L-1, 2.0 mmol·L-1 and 8.0 mmol·L-1 Dio, respectively for 48 h). Cell viability was detected by CCK-8 assay. Apoptosis was detected by Annexin V-FITC/PI staining. The levels of reactive oxygen species (ROS) were measured by fluorescence probe. The level of malondialdehyde (MDA) and the activity of catalase (CAT) and glutathione peroxidase (GSH-Px) were measured by assay kits. Mitochondrial membrane potential was tested by JC-1 staining. The expression levels of B-cell lymphoma/leukaemia-2 (Bcl-2), Bcl-2-associated X protein (Bax), cleaved Caspase-3 and Yes-associated protein (YAP) were tested by Western blot. The expression of YAP was observed by immunofluorescence.Results The screened glucose concentration was 50 mmol·L-1, and the non-cytotoxic dose range of Dio was 0.5 mmol·L-1, 2.0 mmol·L-1 and 8.0 mmol·L-1. Compared with the control group, cell viability, mitochondrial membrane potential, CAT and GSH-Px activity, Bcl-2 and YAP levels in the model group were significantly decreased (all P<0.001), while apoptosis, ROS and MDA levels, cleaved Caspase-3 and Bax levels were significantly increased (all P<0.001). Compared with the model group, cell viability, mitochondrial membrane potential, CAT and GSH-Px activity, Bcl-2 and YAP levels in low-, medium- and high-dose Dio groups were significantly increased, while apoptosis, ROS and MDA levels, cleaved Caspase-3 and Bax levels were significantly decreased, all in a dose-dependent manner(all P<0.05).Conclusion Dio can reduce HG-induced RPE cell injury by activating YAP signal, reducing oxidative stress response and inhibiting mitochondria-mediated apoptosis pathway. |
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| Keywords: | diosgenin diabetic retinopathy retinal pigment epithelium cells high glucose oxidative stress apoptosis |
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