| 细胞因子表达质粒对恶性疟原虫顶端膜抗原1DNA免疫的调节作用 |
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| 引用本文： | 李珣,缪军,雷俊川,薛采芳,王宪锋,刘忠湘,李淑梅.细胞因子表达质粒对恶性疟原虫顶端膜抗原1DNA免疫的调节作用[J].中国寄生虫学与寄生虫病杂志,2004,22(3):136-143. |
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| 作者姓名： | 李珣 缪军 雷俊川 薛采芳 王宪锋 刘忠湘 李淑梅 |
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| 作者单位： | 1. 西藏军区总医院实验中心,拉萨,850003
2. 第四军医大学病,原生物学教研室,西安,710032 |
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| 基金项目： | 军队医药卫生基金 (0 1L0 59)，联合国发展计划署 /世界银行/世界卫生组织热带病研究与培训特别规划 (TDR)项目(A30 1 2 5)~~ |
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| 摘 要： | 目的 探讨细胞因子表达质粒对小鼠DNA免疫的促进和调节作用。 方法 构建编码恶性疟原虫顶端膜抗原1(AMA1)完整胞外域的DNA免疫质粒VR1020/E,构建编码小鼠细胞因子(GMCSF)、白细胞介素(IL)如IL4和IL12的真核表达质粒pcDNA3/GMCSF、pcDNA3.1( ) /IL4和pIL12以及双顺反子质粒pGM CSF/pTPA E,分组免疫小鼠,ELISA检测血清中特异性IgG及其亚类的水平,取小鼠脾细胞进行体外增殖。 结果 3种细胞因子质粒均有效增强了小鼠针对VR10 20/E的免疫应答,抗体水平增加7至10倍,其中pcDNA3/GMCSF质粒和pIL12质粒分别显著促进了小鼠的IgG1和IgG2a应答,小鼠脾细胞的体外增殖水平亦有明显提高。 结论 利用编码GM CSF、IL4和IL12的表达质粒作为佐剂可有效增强小鼠针对AMA1DNA的免疫应答,并对免疫应答的类型产生调节作用。
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| 关 键 词： | 恶性疟原虫 顶端膜抗原1 DNA疫苗 细胞因子类 佐剂 免疫 |
| 文章编号： | 1000-7423(2004)-03-0136-03 |
| 修稿时间： | 2003年9月30日 |
Modulation of Immune Response to Plasmodium falciparum Apical Membrane Antigen 1 DNA Vaccine by Cytokine Plasmids |
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| LI Xun,MIAO Jun,LEI Jun-chuan,XUE Cai-fang,WANG Xian-feng,LIU Zhong-xiang,LI Shu-mei.Modulation of Immune Response to Plasmodium falciparum Apical Membrane Antigen 1 DNA Vaccine by Cytokine Plasmids[J].Chinese Journal of Parasitology and Parasitic Diseases,2004,22(3):136-143. |
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| Authors: | LI Xun MIAO Jun LEI Jun-chuan XUE Cai-fang WANG Xian-feng LIU Zhong-xiang LI Shu-mei |
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| Affiliation: | Research Center, General Hospital of Tibet Military Area, Lasa 850003, China. |
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| Abstract: | Objective To explore the effect of cytokine encoding plasmids on DNA immunization in mice. Methods Prototype DNA vaccine VR1020/E which contain Plasmodium falciparum apical membrane antigen 1 (AMA1) ectodomain was constructed, and eukaryotic expression vectors pcDNA3/GM-CSF, pcDNA3.1(-)/IL-4, pIL-12 and pGM-CSF/pTPA-E were also built. BALB/c mice were immunized with VR1020/E alone or with VR1020/E plus different cytokine plasmids. Serum IgG and its subtype were determined by ELISA and in vitro splenocyte proliferation assay was done. Results GM-CSF, IL-4 and IL-12 encoding plasmids all promoted mice immune response to VR1020/E, the antibody level increased 7 to 10 times and splenocyte proliferation was enhanced too. Plasmid pcDNA3/GM-CSF induced much more IgG1 whereas plasmid pIL-12 induced much more IgG2a. Conclusion Cytokine encoding plasmids might be used as adjuvant in AMA1 DNA immunization. |
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| Keywords: | Plasmodium falciparum Apical membrane antigen 1 DNA vaccine Cytokines Adjuvant Immunologic |
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