| 日本血吸虫分泌蛋白基因Sjsp16的克隆与表达 |
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| 引用本文： | 胡薇,刘锋,王聚君,许学年,韩泽广,冯正.日本血吸虫分泌蛋白基因Sjsp16的克隆与表达[J].中国寄生虫学与寄生虫病杂志,2002,20(5):266-269. |
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| 作者姓名： | 胡薇 刘锋 王聚君 许学年 韩泽广 冯正 |
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| 作者单位： | 1. 中国疾病预防控制中心寄生虫病预防控制所,世界卫生组织疟疾、血吸虫病和丝虫病合作中心,上海,200025
2. 国家人类基因组南方研究中心,上海,201323 |
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| 基金项目： | TMRC NIH USAP50 (AI39461 0 5S1 )，国家高技术研究发展计划课题 (No .2 0 0 1AA2 2 30 91 )~~ |
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| 摘 要： | 目的 克隆和表达日本血吸虫新的分泌蛋白Sjsp16的编码基因。 方法 根据EST测序的结果设计引物 ,从含有Sjsp16基因的cDNA克隆中扩增得到该编码基因 ,亚克隆入原核表达载体 pET2 8中表达 ,然后用生物信息学的方法对蛋白结构功能进行预测。 结果 成功克隆和表达了日本血吸虫分泌蛋白基因Sjsp16,生物信息学分析提示该基因编码蛋白的N端带有一个信号肽序列 ,是一个分泌蛋白 ,含有一个ML功能结构域 ,具有 4种潜在的功能作用位点 ,即 1个N 糖基化位点、3个蛋白激酶C磷酸化位点、4个酪蛋白激酶Ⅱ磷酸化位点和 3个肉豆蔻酰化位点。 结论 Sjsp16基因编码蛋白为具有脂质识别和结合功能的分泌蛋白 ,可能为潜在的血吸虫病药物靶点或疫苗候选分子。
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| 关 键 词： | 日本血吸虫 分泌蛋白 新基因 |
| 文章编号： | 1000-7423(2002)-05-0266-04 |
| 修稿时间： | 2002年8月5日 |
Cloning and Expression of a Secretory Protein Sjsp16 of Schistosoma japonicum |
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| HU Wei,LIU Feng,WANG Ju jun,XU Xue nian,HAN Ze guang,FENG Zheng.Cloning and Expression of a Secretory Protein Sjsp16 of Schistosoma japonicum[J].Chinese Journal of Parasitology and Parasitic Diseases,2002,20(5):266-269. |
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| Authors: | HU Wei LIU Feng WANG Ju jun XU Xue nian HAN Ze guang FENG Zheng |
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| Affiliation: | 1Institute of Parasitic Diseases;Chinese Center for Disease Control and Prevention*;Shanghai 200025; 2National Human Genome Center at Shanghai;Shanghai 201323 |
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| Abstract: | Objective To clone and express a new secretory protein Sjsp16 of Schistosoma japonicum. Methods The specific primers were designed according to the EST sequence and used for amplification of the encoding sequence from the cDNA clone containing Sjsp16. The gene was subcloned into pET28 plasmid and expressed. The structure and functions of this protein were analysed by bioinformatics method. Results The secretory protein gene of Schistosoma japonicum Sjsp16 was cloned and expressed successfully. The bioinformatics analysis showed that there is a signal peptide at the N terminal, which is the character of a secretory protein. It is predicted that Sjsp16 protein contains ML domain, one N glycosylation site, three phosphorylation sites for protein kinase C, four phosphorylation sites for casein kinase II and three N myristoylation sites. Conclusion The protein encoded by Sjsp16 is a secretory one with lipid recognition and lipid binding function, and might be a new candidate for drug target or vaccine. |
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| Keywords: | Schistosoma japonicum secretory protein new gene |
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