| miR-4465调控EZH2的表达抑制胃癌细胞增殖、侵袭和迁移的机制探讨 |
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| 引用本文： | 郑华山,陈成辉,蔡亲平,林献科,李合. miR-4465调控EZH2的表达抑制胃癌细胞增殖、侵袭和迁移的机制探讨[J]. 现代肿瘤医学, 2022, 0(1): 22-27. DOI: 10.3969/j.issn.1672-4992.2022.01.005 |
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| 作者姓名： | 郑华山 陈成辉 蔡亲平 林献科 李合 |
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| 作者单位： | 1.海南医学院第二附属医院东湖外科，海南 海口 570100;2.海南医学院第一附属医院普通外科，海南 海口 570102 |
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| 基金项目： | 海南省重点研发计划(编号:812201)。 |
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| 摘 要： | 目的:探讨miR-4465通过调控EZH2的表达对胃癌细胞增殖、侵袭和迁移的影响。方法:qRT-PCR检测胃癌组织和细胞系中miR-4465的表达水平；在MKN45细胞中过表达miR-4465或沉默EZH2,采用CCK-8和Transwell检测MKN45细胞增殖活力、迁移和侵袭能力；Western blotting检测EZH2、E-cadherin、N-cadherin和Vimentin的表达情况；双荧光素酶报告基因检测miR-4465与EZH2的相互作用。结果:与癌旁组织相比，miR-4465在胃癌组织中的表达降低，在胃癌细胞系(MCC-803、SGC7901、MKN45)中的表达水平显著低于人永生化胃细胞RGM-1,且在MKN45细胞系中的表达低于其他细胞。过表达miR-4465明显抑制MKN45细胞增殖、迁移、侵袭和上皮间质转化(epithelial-mesenchymal transition, EMT);双荧光素酶报告基因结果证实，miR-4465直接靶向作用于EZH2的3’-UTR;进一步实验证明，同时沉默miR-4465和EZH2能够恢复沉默EZH2对MKN45细胞增殖...
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| 关 键 词： | miR-4465 EZH2 胃癌 增殖 迁移 侵袭 |
Study on the mechanism of miR-4465 suppresses the proliferation,migration and invasion of gastric cancer cells by regulating the expression of EZH2 |
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| ZHENG Huashan,CHEN Chenghui,CAI Qinping,LIN Xianke,LI He. Study on the mechanism of miR-4465 suppresses the proliferation,migration and invasion of gastric cancer cells by regulating the expression of EZH2[J]. Journal of Modern Oncology, 2022, 0(1): 22-27. DOI: 10.3969/j.issn.1672-4992.2022.01.005 |
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| Authors: | ZHENG Huashan CHEN Chenghui CAI Qinping LIN Xianke LI He |
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| Affiliation: | 1.Donghu Surgery,the Second Affiliated Hospital of Hainan Medical College,Hainan Haikou 570100,China;2.General Surgery,the First Affiliated Hospital of Hainan Medical College,Hainan Haikou 570102,China. |
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| Abstract: | Objective:To investigate the effect of miR-4465 on the proliferation,migration and invasion of gastric cancer cells by regulating the expression of EZH2.Methods:qRT-PCR was applied to detect the expression level of miR-4465 in gastric cancer tissues and cell lines.Overexpression of miR-4465 or silencing of EZH2 in MKN45 cells,and the proliferation,migration and invasion of MKN45 cells were detected by CCK-8 assay and Transwell assay.Western blotting was used to detect the expression of EZH2,E-cadherin,N-cadherin and Vimentin.The interaction between miR-4465 with EZH2 was verified by dual-luciferase reporter gene.Results:Compared with the adjacent tissues,the expression level of miR-4465 in gastric cancer tissues was decreased,and the expression level in gastric cancer cell lines(MCC-803,SGC7901,MKN45)was significantly lower than that in human immortalized gastric cell RGM-1,and the expression in MKN45 was lower than other cells.Overexpression of miR-4465 significantly inhibits the proliferation,migration,invasion and epithelial-mesenchymal transition of MKN45 cell.Dual-luciferase reporter gene result confirmed that miR-4465 was directly targeted with the 3'-UTR of EZH2.Further experiments showed that simultaneously silencing miR-4465 and EZH2 could restored the inhibitory effect of EZH2 silencing on the proliferation,invasion,migration and EMT of MKN45 cells.Conclusion:miR-4465 is inhibit the biological behavior of gastric cancer cells via targeting EZH2.miR-4465 may be a molecular target for the treatment of gastric cancer. |
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| Keywords: | miR-4465 EZH2 gastric cancer proliferation migration invasion |
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