| 2006-2011年浙江省义乌市鼠疫血清学和病原学动态监测 |
| |
| 引用本文： | 陈劲华,石国祥,翁正军,朱志宏,傅涛,骆淑英. 2006-2011年浙江省义乌市鼠疫血清学和病原学动态监测[J]. 疾病监测, 2012, 27(10): 802-804. DOI: 10.3784/j.issn.1003-9961.2012.10.014 |
| |
| 作者姓名： | 陈劲华 石国祥 翁正军 朱志宏 傅涛 骆淑英 |
| |
| 作者单位： | 1. 义乌市疾病预防控制中心,浙江义乌,322000
2. 浙江省疾病预防控制中心 |
| |
| 摘 要： | 目的 对浙江省义乌市鼠疫历史疫源地宿主和媒介实施鼠疫血清学、病原学及分子生物学动态监测,为制定本地区的鼠疫防控策略提供技术参考。 方法 鼠疫间接血球凝集试验(IHA),鼠疫菌分离培养,聚合酶链反应(PCR)检测鼠疫菌caf1和pla特异性基因。 结果 2006-2011年,共采集鼠形动物血清4207份,IHA检测均呈阴性;动物脏器肝、脾标本各4592份,鼠体蚤115匹,未分离到鼠疫菌;鼠疫菌caf1和pla特异性基因检测931只鼠形动物脏器和115匹鼠体蚤标本结果均呈阴性。 结论 当前义乌市鼠疫历史疫源地未发现鼠间鼠疫疫情迹象。鼠疫监测工作应当进一步深入开展。
|
| 关 键 词： | 间接血球凝集试验 F1抗体 鼠疫菌 特异性基因 鼠疫历史疫源地 |
| 收稿时间： | 2012-04-20 |
Serological and etiological surveillance of plague in Yiwu, Zhejiang ,2006-2011 |
| |
| CHEN Jin-hua ＊,SHI Guo-xiang,WENG Zheng-jun,ZHU Zhi-hong,FU Tao,LUO Shu-ying. Serological and etiological surveillance of plague in Yiwu, Zhejiang ,2006-2011[J]. Disease Surveillance, 2012, 27(10): 802-804. DOI: 10.3784/j.issn.1003-9961.2012.10.014 |
| |
| Authors: | CHEN Jin-hua ＊ SHI Guo-xiang WENG Zheng-jun ZHU Zhi-hong FU Tao LUO Shu-ying |
| |
| Affiliation: | . ＊ Yiwu Center for Disease Control and Prevention, Yiwu 322000, Zhejiang, China |
| |
| Abstract: | Objective To understand the distribution of host animals and vectors of plague in historic plague focus in Yiwu, Zhejiang province, and provide evidence for the development of local plague prevention and control strategies. Methods Hague pathogen isolation and indirect hemagglutination assay （IHA） were conducted, carl and pla, the specific genes of Yersinia pestis, were detected by polymerase chain reaction （PCR）. Results From 2006 to 2011, 4207 serum samples collected from roden-like animals were detected and the IHA results were all were negative. No plague pathogen was isolated from 4592 liver samples and 4592 spleen samples of rodent-like animals and 115 flea samples taken from rats. The PCR detection results of caf1 and pla genes in 931 rodent-like animal organs and 115 flea samples were negative too. Conclusion No sign of rat plague was found in the historic plague focus in Yiwu, but it is still necessary to continue the surveillance of plague there. |
| |
| Keywords: | indirect hemagglutination assay F1 antibody Yersinia pestis specific gene historic plague focus |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《疾病监测》浏览原始摘要信息 |
|
点击此处可从《疾病监测》下载免费的PDF全文 |
|
