| 再治疗根管粪肠球菌生物膜形成与临床表现相关性分析 |
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| 作者姓名： | Zheng J Wang L Song Q Li J Niu W |
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| 作者单位： | 1.大连医科大学口腔医学院口腔内科教研室, 大连116044; 2.口腔疾病研究国家重点实验室, 四川大学; 3.四川大学华西口腔医院牙体牙髓病科, 成都610041 |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 目的检测粪肠球菌形成生物膜的能力,探讨其生物膜形成能力与临床表现之间的关系。方法采用96孔板法形成生物膜,结合结晶紫染色,检测临床样本中分离的53株粪肠球菌形成生物膜的能力,分析其生物膜形成能力与患牙临床表现之间的关系。结果53株粪肠球菌中,40株（75.47%）具有生物膜形成能力;在患牙的多种临床表现中,瘘道与再治疗根管粪肠球菌生物膜形成具有相关性,结果具有统计学意义（P＜0.05）。结论再治疗根管中,无瘘道的患牙分离出来的粪肠球菌生物膜形成能力强于有瘘道的患牙,临床治疗中应予以注意。
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| 关 键 词： | 粪肠球菌 根管再治疗 生物膜 |
| 收稿时间： | 2012-02-25 |
| 修稿时间： | 2012-02-25 |
The correlation analysis on Enterococcus faecalis biofilm and clinical manifestation in retreatment of root canal |
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| Zheng J,Wang L,Song Q,Li J,Niu W.The correlation analysis on Enterococcus faecalis biofilm and clinical manifestation in retreatment of root canal[J].West China Journal of Stomatology,2012,30(1):65-67. |
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| Authors: | Zheng Jianbo Wang Lina Song Qiyi Li Jiyao Niu Weidong |
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| Affiliation: | 1. Dept. of Oral Medicine, School of Stomatology, Dalian Medical University, Dalian 116044, China; 2. State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610041, China; 3. Dept. of Endodontics, West China School of Stomatology, Sichuan University, Chengdu 610041, China |
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| Abstract: | Objective To detect the ability of Enterococcus faecalis(E.faecalis) biofilm formation and explore the relationship between E.faecalis biofilm formation ability and clinical manifestation.Methods 96 well plate with the establishment of 53 E.faecalis in vitro biofilm model,combined with crystal violet staining,was used to test the bio-film formation ability of the clinical isolates E.faecalis and analyze the relationship between biofilm formation capacity and clinical manifestation.Results In total 53 E.faecalis strains,40 strains(75.47%) had biofilm forming ability.Stati-stical analysis revealed that the capacities of biofilm formation between E.faecalis isolated from with fistula and without fistula was significantly different(P<0.05).Conclusion In the retreatment of root canal,the ability of biofilm formation of E.faecalis separated from the teeth without fistula is better than those separated from the teeth with fistula. |
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| Keywords: | Enterococcus faecalis root canal retreatment biofilm |
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