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| 感染细粒棘球蚴绵羊诱发过敏性休克期间IgG、IgG1和IgE水平的探讨 | |
| 引用本文： | 郑宏,徐志新,杨戈雄,温浩.感染细粒棘球蚴绵羊诱发过敏性休克期间IgG、IgG1和IgE水平的探讨[J].中国寄生虫学与寄生虫病杂志,2003,21(1):42-45. |
| 作者姓名： | 郑宏 徐志新 杨戈雄 温浩 |
| 作者单位： | 1. 新疆医科大学第一附属医院麻醉科,乌鲁木齐,830054 2. 武警新疆总队医院麻醉科,乌鲁木齐,830091 3. 新疆医科大学第一附属医院普外科,乌鲁木齐,830054 |
| 基金项目： | 国家自然科学基金 (No.39860 0 78)~~ |
| 摘 要： | 目的 测定感染细粒棘球蚴(E.g)绵羊诱发过敏性休克期间特异性IgG、IgG1和IgE抗体水平。了解抗原B对人工感染E.g绵羊IgG抗体的反应性。 方法 从绵羊E.g囊液中制备抗原B和E.g囊液粗制抗原,ELISA测定感染E.g绵羊诱发休克期间特异性IgG、IgG1和IgE抗体的动态变化。 结果 感染E.g绵羊6个月,特异性IgG、IgG1和IgE抗体水平较正常绵羊显著升高;诱发休克后特异性IgE抗体水平显著下降,尤其因休克致死的绵羊下降更为明显;IgG及IgG1抗体的衰减时间不同,趋势各不相同;抗原B和E.g囊液粗制抗原与血清IgG抗体反应阳性率分别为91%、32%。 结论 特异性IgE是导致棘球蚴病所致过敏性休克的主要抗体,而IgG和IgG1抗体也起着重要作用。抗原B与感染E.g绵羊IgG抗体的血清反应性较好,可作为一种血清免疫学诊断方法监测绵羊感染E.g的状况。 |
| 关 键 词： | 细粒棘球蚴 绵羊 过敏性休克 抗体 抗原B |
| 文章编号： | 1000-7423(2003)-01-0042-04 |
| 修稿时间： | 2002年1月14日 |
Study on the Level of Specific IgG, IgG1 and IgE During Anaphylactic Shock in Sheep Induced by Echinococcus granulosus |
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| ZHENG Hong,XU Zhi-xinYang Ge-xiong,WEN Hao.Study on the Level of Specific IgG, IgG1 and IgE During Anaphylactic Shock in Sheep Induced by Echinococcus granulosus[J].Chinese Journal of Parasitology and Parasitic Diseases,2003,21(1):42-45. | |
| Authors: | ZHENG Hong XU Zhi-xinYang Ge-xiong WEN Hao |
| Affiliation: | Department of Anesthesiology, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054. |
| Abstract: | Objective To investigate the change of specific antibodies IgG, IgG1 subclass and IgE in sheep infected with Echinococcus granulosus(E.g) during anaphylactic shock, and to observe antigen B reactivity against IgG antibody in E.g\|infected sheep. \ Methods\ Antigen B and crude antigen were prepared with E.g cyst fluid (EgCF) from infected sheep. The enzyme\|linked immunosorbent assay(ELISA) was used for detecting the level of specific IgG, IgG1 and IgE during anaphylactic shock in sheep induced by E.g. \ Results \ The level of specific IgG, IgG1 and IgE was significantly higher in the infected sheep after 6 months than that of the uninfected control group (P<0\^01). The IgE level decreased rapidly after anaphylactic shock induced, especially when the sheep was dying. Following an antigen challenge the sheep showed a general decrease in total IgG and IgG1 subclass. The total IgG showed a slight change at the beginning,followed by a decrease 1 h after challenge. The decrease of IgG1 subclass was more significant than the total IgG in 40 min after challenge injection. The positive rate was 91% for antigen B and 32% for crude antigen of EgCF against IgG antibody in E.g\|infected sheep. \ Conclusion \ The specific IgE plays a major role in the anaphylactic shock, while IgG and IgG1 \{antibodies\} are also important. Antigen B derived from sheep E.g cyst fluid appears to be useful in serodiagnosis of and \{monitoring\} on the infection status in sheep. |
| Keywords: | Echinococcus granulosus sheep anaphylactic shock antibody Antigen B |
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