| 马齿苋多糖对胃癌SGC7901细胞增殖和凋亡的影响 |
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| 引用本文： | 欧海玲,张秀玲,孙平良,张锡流,耿曙光,覃宇周. 马齿苋多糖对胃癌SGC7901细胞增殖和凋亡的影响[J]. 中国癌症防治杂志, 2020, 12(4): 431-434. DOI: 10.3969/j.issn.1674-5671.2020.04.12 |
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| 作者姓名： | 欧海玲 张秀玲 孙平良 张锡流 耿曙光 覃宇周 |
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| 作者单位： | 广西中医药大学第一附属医院病理科；广西中医药大学第一附属医院仙葫院区普外科；广西医科大学附属肿瘤医院胃肠外科 |
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| 基金项目： | 广西中药药效研究重点实验室开放课题（11-031-05-k4） |
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| 摘 要： | 目的 探讨马齿苋多糖对胃癌SGC7901细胞增殖和凋亡的影响。方法 体外培养胃癌SGC7901细胞，分别用不同浓度（25 μg/mL、50 μg/mL、100 μg/mL、200 μg/mL、300 μg/mL）马齿苋多糖作用胃癌SGC7901细胞，以生理盐水溶液为阴性对照组，5-氟尿嘧啶（5-FU）为阳性对照组，采用MTT法检测细胞增殖情况，流式细胞仪检测细胞凋亡情况。结果 不同浓度马齿苋多糖作用胃癌SGC7901细胞的增殖抑制率随着作用时间的延长和药物浓度的提高而增加。作用48 h后，200 μg/mL、300 μg/mL马齿苋多糖组的细胞增殖抑制率分别为（53.02±2.46）%、（57.20±2.14）%，与5-FU组细胞增殖抑制率（56.36±3.26）%相当（P＞0.05）；各浓度组的细胞凋亡率依次为31.92%、38.39%、40.45%、48.49%、55.71%，与阴性对照组比较均升高（均P＜0.05）；与5-FU组比较，低浓度（25 μg/mL、50 μg/mL、100μg/mL）组的细胞凋亡率降低（P＜0.05），而高浓度（300 μg/mL）组的细胞凋亡率升高（P＜0.05）。结论 马齿苋多糖可抑制胃癌SGC7901细胞增殖并诱导其凋亡。
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Effects of portulaca oleracea polysaccharide on the proliferation and apoptosis of gastric cancer SGC7901 cells |
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| OU Hailing,ZHANG Xiuling,SUN Pingliang,ZHANG Xiliu,GENG Shuguang,QIN Yuzhou. Effects of portulaca oleracea polysaccharide on the proliferation and apoptosis of gastric cancer SGC7901 cells[J]. Journal of Chinese Medical Abstracts·Oncology, 2020, 12(4): 431-434. DOI: 10.3969/j.issn.1674-5671.2020.04.12 |
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| Authors: | OU Hailing ZHANG Xiuling SUN Pingliang ZHANG Xiliu GENG Shuguang QIN Yuzhou |
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| Abstract: | Objective To investigate the effects of portulaca oleracea polysaccharide on the proliferation and apoptosisof gastric cancer SGC7901 cells. Methods Gastric cancer SGC7901 cells were cultured in vitro and treated with differentconcentrations of portulaca oleracea polysaccharides（25 μg/mL，50 μg/mL，100 μg/mL，200 μg/mL，300 μg/mL）. Normalsaline was used as the negative control group and 5-FU as the positive control group. The cell proliferation was detectedby MTT assay，and apoptosis was detected by flow cytometry. Results The proliferation inhibition rate of SGC7901 cellstreated with portulaca oleracea polysaccharide at different concentrations increased with the increases of duration timeand drug concentration. After 48 h，the proliferation inhibition rates of 200 μg/mL and 300 μg/mL portulaca oleracea polysaccharide groups were（53.02±2.46）%，（57.20±2.14）%，respectively，which were comparable to those of the 5-FU group，（56.36±3.26）% （P>0.05）. The apoptosis rates of each concentration group were significantly increased compared with the negative control group，followed by 31.92%，38.39%，40.45%，48.49%，55.71%（all P<0.05）. Compared with the 5-FU group，the apoptosis rates of low-concentration（25 μg/mL，50 μg/mL，and 100 μg/mL） groups were decreased（P<0.05）， while the apoptosis rate was increased in the high concentration（300 μg/mL） group（P<0.05）. Conclusions Portulaca oleracea polysaccharide can inhibit the proliferation and induce the apoptosis of gastric cancer SGC7901 cells. Portulaca oleracea polysaccharide can inhibit the proliferation and induce the apoptosis of gastric cancer SGC7901 cells. |
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| Keywords: | Gastric cancer Portulaca oleracea polysaccharide Proliferation Apoptosis |
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