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| 川桑中桑皮素的提取工艺优化 | |
| 作者姓名： | 左亚奇 高志红 庞策 孙转友 李芳 甄攀 |
| 作者单位： | 1. 河北北方学院，张家口，075000，中国 2. 广宗县中医医院，邢台，054600，中国 3. 北京中医药大学东方学院，廊坊，065000，中国 |
| 基金项目： | 河北省高等学校科学技术研究重点项目（No：ZD2016016） |
| 摘 要： | 目的：探讨川桑中桑皮素的最佳提取工艺。方法：以桑皮素含量为指标，利用4 因素3 水平L9（34）正交试验，分析乙醇浓度、提取时间、溶媒倍数、提取次数对桑皮素提取效率的影响。HPLC 法测定桑皮素含量：YMCPack ODS-A 色谱柱（5 μm，4.6 mm×250 mm）；流动相为甲醇- 水（80：20）；柱温25 ℃；流速1.0 mL·min-1；检测波长268 nm。结果：最佳提取工艺：提取前提取溶剂浸泡3 h，回流提取3 次，每次提取时间均为0.5 h，提取溶媒倍数均为20 倍。最优提取条件下测得川桑中桑皮素含量为1.510 mg·g-1。结论：乙醇浓度、溶媒倍数、提取次数对桑皮素提取效率均有显著影响，提取时间无显著影响。 |
| 关 键 词： | 正交试验 川桑 桑皮素 提取工艺 HPLC |
Optimized Extraction of Mulberrin from Morus Notabilis Schneid |
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| Authors: | ZUO Ya-qi GAO Zhi-hong PANG Ce SUN Zhuan-you LI Fang ZHEN Pan |
| Affiliation: | 1. Hebei North University，Zhangjiakou，075000，China 2. Guangzong County Hospital of Traditional Chinese Medicine，Xingtai，054600，China 3. Beijing University of Chinese Medicine DongFang Colege，Langfang，065000，China |
| Abstract: | Objective：To investigate the optimum extraction technology of mulberrin from Morus notabilis Schneid. Methods：Taking the content of mulberrin as an index，the effects of ethanol concentration，extraction time，solvent multiple，extraction times on the extraction efficiency of mulberrin was investigated by 4-Factor-3-Level orthogonal experiment. HPLC was used to detect the extraction efficiency of mulberrin on YMC-Pack ODS-A column （4.6 mm×250 mm，5 μm） with water- methanol （20：80） as mobile phase. The flow rate was 1.0 mL·min-1，the column temperature was 25 ℃ and the wavelength of UV detector was 268 nm. Results：The optimum extraction technology：before extraction，Morus notabilis was soaked in extraction solvent for 3.0 h，and extracted with reflux for 3 times，each extraction time was 0.5 h，and the extraction solvent multiple was 20 times. Under optimal extraction conditions，the content of mulberry in Morus notabilis Schneid was 1.510 mg·g-1. Conclusion：Ethanol concentration，solvent multiple and extraction times all had significant effects on the extraction efficiency of mulberry，and the extraction time didn’t have significant influences on the extraction efficiency of mulberry. |
| Keywords: | orthogonal experiment Morus notabilis Schneid mulberrin extraction process HPLC |
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