Agents That Inhibit Rho, Rac, and Cdc42 Do Not Block Formation of Actin Pedestals in HeLa Cells Infected with Enteropathogenic Escherichia coli |
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| Authors: | Goni Ben-Ami Vered Ozeri Emanuel Hanski Fred Hofmann Klaus Aktories Klaus M. Hahn Gary M. Bokoch Ilan Rosenshine |
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| Affiliation: | Departments of Molecular Genetics and Biotechnology1. and of Clinical Microbiology,2. Faculty of Medicine, The Hebrew University, Jerusalem 91120, Israel; Institute of Pharmacology and Toxicology, Albert-Ludwigs University, Freiburg, Germany3.; and Departments of Cell Biology4. and of Immunology,5. The Scripps Research Institute, La Jolla, California 92037 |
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| Abstract: | Enteropathogenic Escherichia coli (EPEC) induces formation of actin pedestals in infected host cells. Agents that inhibit the activity of Rho, Rac, and Cdc42, including Clostridium difficile toxin B (ToxB), compactin, and dominant negative Rho, Rac, and Cdc42, did not inhibit formation of actin pedestals. In contrast, treatment of HeLa cells with ToxB inhibited EPEC invasion. Thus, Rho, Rac, and Cdc42 are not required for assembly of actin pedestals; however, they may be involved in EPEC uptake by HeLa cells. |
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