| 肺癌A549细胞株中ALDH1+细胞的生物学功能研究 |
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| 引用本文： | 邹爱梅,吴爱兵,黄维甄,张金标,谢剑明,李荣,罗荣城.肺癌A549细胞株中ALDH1+细胞的生物学功能研究[J].中国肿瘤临床,2011,38(14):817-821. |
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| 作者姓名： | 邹爱梅 吴爱兵 黄维甄 张金标 谢剑明 李荣 罗荣城 |
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| 作者单位： | 南方医科大学南方医院肿瘤中心(广州市510515) |
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| 摘 要： | 探讨肺癌A549细胞株中具有肿瘤干细胞特性的ALDH1+细胞的生物学功能特性。方法:运用流式细胞仪分选肺癌A549细胞株中ALDH1+细胞，并通过肿瘤球培养、MTT实验、平板克隆、Transwell小室迁移实验、裸鼠体内成瘤实验来验证ALDH1+细胞的自我更新、增殖克隆、迁移及致瘤能力。结果:ALDH1+ A549细胞的肿瘤球形成能力、增殖克隆、迁移和体内成瘤能力明显高于ALDH1- A549细胞和未经分选的A549细胞。结论:A549细胞株中ALDH1+ A549细胞具有自我更新、增殖、迁移和高致瘤能力的肿瘤干细胞特性，ALDH1可作为分选肺癌肿瘤干细胞的标志物。
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| 关 键 词： | ALDH1 肺癌 肿瘤干细胞 肿瘤球 |
| 收稿时间： | 2011-03-05 |
Biological Functions of ALDH1-positive Cancer Cells in the A549 Lung Cancer Cell Line |
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| Aimei ZOU,Aibing WU,Weizhen HUANG,Jinbiao ZHANG,Jianming XIE,Rong LI,Rongcheng LUO.Biological Functions of ALDH1-positive Cancer Cells in the A549 Lung Cancer Cell Line[J].Chinese Journal of Clinical Oncology,2011,38(14):817-821. |
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| Authors: | Aimei ZOU Aibing WU Weizhen HUANG Jinbiao ZHANG Jianming XIE Rong LI Rongcheng LUO |
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| Affiliation: | Department of Oncology, Nanfang Hospital, Nanfang Medical University, Guangzhou 510515, China |
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| Abstract: | To investigate the biological functions of the ALDH1-positive cancer cells exhibiting the characteristics of a cancer stem cell in the A549 lung cancer cell line. Methods: Fluorescence-activated cell sorting? analysis was used to differentiate between the ALDH1-positive and ALDH1-negative cancer cells in an A549 lung cancer cell line. Tumor sphere cultivation, MTT, clonogenicity, and in vitro transwell assays as well as tumor initiation in vivo were used to compare the self-renewing, proliferative, clonogenic, and invasive abilities as well as the tumorigenicity between the ALDH1-positive, ALDH1-negative, and unsorted A549 cells. Results: The self-renewing, proliferative, clonogenic, and invasive abilities as well as the tumorigenicity of the ALDH1-positive cancer cells in the A549 lung cancer cell line were higher than those of the ALDH1-negative and unsorted A549 cells. Conclusion: The ALDH1-positive cancer cells in the A549 lung cancer cell line possess the characteristics of cancer stem cells and may be used as NSCLCSC-associated tumor markers. |
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| Keywords: | ALDH1 |
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