Molecular diagnosis of Strongyloides stercoralis among transplant candidates |
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| Authors: | Fabiana M. Paula Fernanda M. Malta Priscilla D. Marques Gessica B. Melo Marcelo A. Corral Maiara Gottardi João R. R. Pinho Elenice M. N. Gonçalves Vera L. P. Castilho Ligia C. Pierrotti Edson Abdala Silvia F. Costa Pedro P. Chieffi Ronaldo C. B. Gryschek |
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| Affiliation: | 1. Laboratório de Investiga??o Médica (LIM/06 – Laboratório de Imunopatologia da Esquistossomose), Hospital das Clínicas da Faculdade de Medicina, USP, S?o Paulo, Brazil;2. Instituto de Medicina Tropical de S?o Paulo, USP, S?o Paulo, Brazil;3. Laboratório de Investiga??o Médica (LIM/07 – Laboratório de Gastroenterologia e Hepatologia Tropical), Hospital das Clínicas da Faculdade de Medicina, USP, S?o Paulo, Brazil;4. Se??o de Parasitologia da Divis?o de Laboratório Central, Hospital das Clínicas da Faculdade de Medicina, USP, S?o Paulo, Brazil;5. Faculdade de Medicina, USP, S?o Paulo, Brazil;6. Faculdade de Ciências Médicas, Santa Casa, S?o Paulo, Brazil |
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| Abstract: | Strongyloidiasis can occur without any symptoms or as a potentially fatal hyperinfection or disseminated infection, principally in immunosuppressed patients. Our study aimed to evaluate the application of conventional polymerase chain reaction (cPCR) and real‐time PCR (qPCR). Polymerase chain reaction (PCR) and real‐time PCR (qPCR) targeting the 18S rRNA gene for detection of Strongyloides stercoralis infection among transplant candidates were applied in stool samples obtained from 150 transplant candidates, preliminarily analyzed by parasitological methods. S. stercoralis larvae were visualized in 15/150 (10.0%) transplant candidates by parasitological methods. DNA from S. stercoralis was amplified in 26/150 (17.3%) and 49/150 (32.7%) stool samples of transplant candidates, using cPCR and qPCR, respectively. The results suggest that molecular methods, especially qPCR, should be used as an additional tool for diagnostic of S. stercoralis infection among transplant candidates. |
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| Keywords: | molecular diagnosis
Strongyloides stercoralis
transplant candidate |
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