| miR-140-5p靶向调控VEGFA参与卵巢癌血管生成的作用机制 |
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| 引用本文： | 阎臻,付晓瑞,李新敏,崔珺.miR-140-5p靶向调控VEGFA参与卵巢癌血管生成的作用机制[J].现代肿瘤医学,2021,0(7):1118-1123. |
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| 作者姓名： | 阎臻 付晓瑞 李新敏 崔珺 |
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| 作者单位： | 1.郑州市妇幼保健院妇产科，河南 郑州 450052;
2.郑州大学第一附属医院肿瘤科，河南 郑州 450052;
3.郑州市妇幼保健院病理科，河南 郑州 450052 |
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| 基金项目： | 河南省卫计委普通攻关项目(编号:201503211)。 |
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| 摘 要： | 目的:探究miR-140-5p靶向调控VEGFA基因参与卵巢癌血管生成的作用机制。方法:采集实验组织标本和细胞系，qRT-PCR检测组织和细胞中miR-140-5p和VEGFA的表达水平。Western blot检测VEGFA及血管生成相关因子STAT3、HIF-1α和bFGF的表达。应用流式细胞术检测转染细胞凋亡情况。结果:卵巢癌组织和细胞中miR-140-5p低表达，VEGFA为高表达（均P＜0.05）。荧光素酶报告法确认VEGFA为miR-140-5p靶点。上调miR-140-5p表达，可降低卵巢癌细胞株CAOV3中VEGFA表达，下调miR-140-5p表达，可诱导VEGFA表达（均P＜0.05）。与mimic NC+VEGFA-NC组相比，miR-140-5p mimic+VEGFA-NC组miR-140-5p表达量增加（P＜0.05），而VEGFA mRNA和蛋白表达量变化未达明显统计学差异（均P＞0.05）；与miR-140-5p mimic+VEGFA-NC组相比，miR-140-5p mimic+VEGFA-siRNA组miR-140-5p表达量显著增加，VEGFA的mRNA表达量显著下降（P＜0.05），且STAT3、HIF-1α、bFGF mRNA和蛋白表达量均显著下降（均P＜0.05）。同时，与mimic NC+VEGFA-NC组和miR-140-5p mimic+VEGFA-NC组相比，miR-140-5p mimic+VEGFA-siRNA组细胞凋亡率明显增加，差异有显著统计学意义（P＜0.05）。结论:miR-140-5p在卵巢癌中低表达，VEGFA高表达，miR-140-5p能特异结合VEGFA基因；上调miR-140-5p表达靶向下调VEGFA表达可降低卵巢癌血管生成相关因子表达并诱导癌细胞凋亡，为卵巢癌分子靶向治疗提供生物学证据。
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| 关 键 词： | miR-140-5p VEGFA 卵巢癌 血管生成 细胞凋亡 |
Mechanism of microRNA-140-5p in angiogenesis of ovarian cancer by targeting the regulation of VEGFA |
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| YAN Zhen,FU Xiaorui,LI Xinmin,CUI.Mechanism of microRNA-140-5p in angiogenesis of ovarian cancer by targeting the regulation of VEGFA[J].Journal of Modern Oncology,2021,0(7):1118-1123. |
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| Authors: | YAN Zhen FU Xiaorui LI Xinmin CUI |
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| Affiliation: | 1.Department of Obstetrics and Gynecology,Women & Infants Hospital of Zhengzhou,Henan Zhengzhou 450052,China;2.Department of Oncology,First Affiliated Hospital of Zhengzhou University,Henan Zhengzhou 450052,China;3.Pathology Department,Women & Infants Hospital of Zhengzhou,Henan Zhengzhou 450052,China. |
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| Abstract: | Objective:To explore the mechanism of miR-140-5p targeting the regulation of vascular endothelial growth factor A(VEGFA)gene in ovarian cancer angiogenesis.Methods:After collecting experimental tissue specimens and cell lines,the expression levels of miR-140-5p and VEGFA in tissues and cells were detected by qRT-PCR,followed by the detection of the expression of VEGFA,as well as other angiogenesis related factors STAT3,HIF-1αand bFGF by Western blot,and the evaluation of the apoptosis of transfected cells by flow cytometry.Results:The expression of miR-140-5p was low in ovarian cancer tissues and cells,while the expression of VEGFA was high(All P<0.05).Luciferase reporter assay confirmed that VEGFA was a target of miR-140-5p.Up-regulation of the expression of miR-140-5p can reduce the expression of VEGFA in ovarian cancer cell line CAOV3,while down-regulation of the expression of miR-140-5p can induce the expression of VEGFA(All P<0.05).The expression of miR-140-5p in miR-140-5p mimic+VEGFA-NC group increased compared with mimic NC+VEGFA-NC group(P<0.05),yet no obvious difference was found in the mRNA and protein expression of VEGFA(All P>0.05).Compared with the expression in miR-140-5p mimic+VEGFA-NC group,the expression of miR-140-5p increased significantly and the expression of VEGFA decreased significantly in the miR-140-5p mimic+VEGFA-siRNA group(P<0.05),and the protein expression of STAT3,HIF-1αand bFGF were significantly decreased(All P<0.05).Meanwhile,compared with mimic NC+VEGFA-NC group and miR-140-5p mimic+VEGFA-NC group,the apoptotic rate increased in miR-140-5p mimic+VEGFA-siRNA group remarkably,showing statistical difference(P<0.05).Conclusion:The expression of miR-140-5p is low in ovarian cancer,and the expression of VEGFA is high,and miR-140-5p can specifically bind to VEGFA.Up-regulation of VEGFA expression and down-regulation of targeted expression of VEGFA can reduce the expression of angiogenesis-related factors in ovarian cancer and promote the apoptosis of ovarian cancer cells,providing biological evidence for molecular targeted therapy of ovarian cancer. |
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| Keywords: | miR-140-5p VEGFA ovarian cancer angiogenesis cell apoptosis |
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