| HSV-TK/GCV系统在体外对胃癌MNK-45细胞株的增殖抑制作用研究 |
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| 引用本文： | 李娅,张赟,刘红莉,皇海,苏明权,屈慧.HSV-TK/GCV系统在体外对胃癌MNK-45细胞株的增殖抑制作用研究[J].现代检验医学杂志,2021,0(2):19-23. |
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| 作者姓名： | 李娅 张赟 刘红莉 皇海 苏明权 屈慧 |
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| 作者单位： | （1.西安市人民医院（西安市第四医院）检验科，西安 710004; 2.空军军医大学第一附属医院全军临床检验医学研究所，西安710032） |
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| 基金项目： | 陕西省科技厅一般项目-社会发展领域(2017SF-265),西安市人民医院(西安市第四医院)孵化基金CX-20。 |
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| 摘 要： | 目的 探讨单纯疱疹病毒胸苷激酶基因/丙氧鸟苷（HSV-TK/GCV）自杀基因系统对胃癌MNK-45细胞增殖、侵袭和迁移的抑制效应。方法 分别采用MTS试验、CCK8试验、Transwell试验、Western blot试验和RT-PCR方法检测HSV-TK/GCV系统对胃癌MNK-45细胞在24，48，72和96h的增殖、侵袭和迁移的改变；对MNK-45细胞凋亡相关蛋白mRNA水平的变化。结果 HSV-TK/GCV系统能够有效抑制胃癌MNK-45细胞的增殖效应，并能够减弱胃癌MNK-45细胞的侵袭和迁移能力，诱导MNK-45细胞凋亡相关蛋白Caspase3、Bax蛋白水平和mRNA的表达增加，抑制Bcl-2蛋白表达水平的降低，促进凋亡的发生。结论 HSV-TK/GCV系统能够有效抑制胃癌MNK-45细胞增殖、侵袭和迁移，诱导MNK-45细胞凋亡的发生。
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| 关 键 词： | 胃癌 单纯疱疹病毒胸苷激酶基因/丙氧鸟苷系统 MNK-45细胞 细胞增殖 |
Study on the Inhibition of HSV-TK/GCV System on the Proliferation of Gastric Cancer MNK-45 Cell Line in Vitro |
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| LI Ya,ZHANG Yun,LIU Hong-li,HUANG Hai,SU Ming-quan,QU Hui.Study on the Inhibition of HSV-TK/GCV System on the Proliferation of Gastric Cancer MNK-45 Cell Line in Vitro[J].Journal of Modern Laboratory Medicine,2021,0(2):19-23. |
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| Authors: | LI Ya ZHANG Yun LIU Hong-li HUANG Hai SU Ming-quan QU Hui |
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| Affiliation: | [1.Departments of Clinical Laboratory, Xi’an People’s Hospital（Xi’an Fourth Hospital）, Xi’an 710004, China; 2. Institute of Clinical Laboratory Medicine of People’s Liberation Army, the First Affiliated Hospital of AFMU, Xi’an 710032, China] |
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| Abstract: | Objective To investigate the inhibitory effect of HSV-TK/GCV suicide gene system on proliferation,invasion and migration of MNK-45 cells in gastric cancer.Methods MTS test,CCK8 test,Transwell test,Western blot test and RT-PCRwere used to detect the proliferation of MNK-45 cells in gastric cancer at 24,48,72 and 96h by HSV-TK/GCV.Transwell test was used to detect the invasion and migration of MNK-45 cells in gastric cancer by HSV-TK/GCV system.Changes of mRNA levels of apoptosis-related proteins in MNK-45 cells.Results HSV-TK/GCV system can effectively inhibit the proliferation effect of grastic cancer MNK-45 cells,reduce the invasion and migration ability of grastic cancer MNK-45 cells,induce the expression level and mRNA of apoptosis-related proteins Caspase 3 and Bax,inhibit the expression level of Bcl-2,and promote the occurrence of apoptosis.Conclusion HSV-TK/GCV system can effectively inhibit the proliferation,invasion and migration of grastic cancer MNK-45 cells and induce the occurrence of apoptosis of MNK-45 cells. |
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| Keywords: | gastric cancer HSV-TK/GCVsystem MNK-45 cells cell proliferation |
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